质粒载体 - Biovector质粒载体菌种细胞蛋白抗体基因保藏中心-NTCC典型培养物保藏中心

Plasmids pZS*13S pSC101 origin, AmpR, PA1lacO-1 pZA33S P15A origin, ChlorR, PA1lacO-1 pZE13S ColE1 origin, AmpR, PA1lacO- pZE23S ColE1 origin, KanR, PA1lacO-1

Biovector

¥4950

2017-02-21

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pHLSec2-FN-YPet表达质粒Plasmid 65421

pHLSec2-FN-YPet Plasmid 65421Map:PURPOSEExpresses in mammalian cells human plasma fibronectin with YPet inserted betwe...

Biovector

¥9830

2017-02-21

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pET6xHN-N, pET6xHN-C, pET6xHN-GFPuv Vector原核6xHN标签表达载体及原核荧光表达载体-BioVector保藏中心

pET6xHN-N, pET6xHN-C, and pET6xHN-GFPuv Vector原核6xHN标签表达载体及原核荧光表达载体描述：T7 lac启动子，6xHN标签，GFPuv荧光蛋...

Biovector

¥7930

2017-02-21

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pCAMBIA1305.1植物荧光表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pCAMBIA1305.1植物荧光表达载体Map图谱：Sequence序列：CATGGTAGATCTGAGGGTAAATTTCTAGTTTTTCTCCTTCATTTTCTTGGTTAGGACCCTTTTCTCTTTTTATTTTTT...

Biovector

¥14930

2017-02-21

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pBT3-STE酵母双杂交系统载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pBT3-STE酵母双杂交系统载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心Map图谱：

Biovector

¥8930

2017-02-22

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HIV-1 Gag-mCherry荧光表达质粒pcDNA3.1/Zeo (+)，85390-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

HIV-1 Gag-mCherry Plasmid 85390 Gag fragment was amplified from p96ZM651gag-opt vector [NIH AIDS Research and Reference Reagent Program] and cloned into pcDNA3.1/Zeo(+) using HindIII and BamHI sites. mCherry fragment was amplified from pRSET-BmCherry and cloned into pcDNA3.1/Zeo(+) using BamHI and XhoI sites. HIV-1 Gag-mCherry.png BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 PURPOSE Visualization marker for viral core to measure cellular uptake Vector backbonepcDNA3.1/Zeo (+) Backbone manufacturerInvitrogen Backbone size w/o insert (bp)5015 Vector typeMammalian Expression Selectable markersZeocin GROWTH IN BACTERIA Bacterial Resistance(s)Ampicillin Growth Temperature37°C Growth Strain(s)DH5alpha Copy numberHigh Copy GENE/INSERT Gene/Insert nameHIV-1 gag SpeciesHuman immunodeficiency virus 1 Insert Size (bp)2214 PromoterCMV Tag / Fusion Protein mCherry (C terminal on insert) CLONING INFORMATION Cloning methodRestriction Enzyme 5′ cloning siteHindIII (not destroyed) 3′ cloning siteXhoI (not destroyed) 5′ sequencing primerCMV-F 3′ sequencing primerBGH-rev Sequence: >HIV-1 Gag-mCherry sequence 7167 bps GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTT AAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACA ACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCG ATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTC ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC ATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTA TGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGC AGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACG CAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCA CTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTT AAGCTTGCCACCATGGGCGCCCGCGCCAGCGTGCTGAGCGGCGGCGAGCTGGACAAGTGGGAGAAGATCC GCCTGCGCCCCGGCGGCAAGAAGAAGTACAAGCTGAAGCACATCGTGTGGGCCAGCCGCGAGCTGGAGCG CTTCGCCGTGAACCCCGGCCTGCTGGAGACCAGCGAGGGCTGCCGCCAGATCCTGGGCCAGCTGCAGCCC AGCCTGCAGACCGGCAGCGAGGAGCTGCGCAGCCTGTACAACACCGTGGCCACCCTGTACTGCGTGCACC AGCGCATCGACGTCAAGGACACCAAGGAGGCCCTGGAGAAGATCGAGGAGGAGCAGAACAAGTCCAAGAA GAAGGCCCAGCAGGCCGCCGCCGCCGCCGGCACCGGCAACAGCAGCCAGGTGAGCCAGAACTACCCCATC GTGCAGAACCTGCAGGGCCAGATGGTGCACCAGGCCATCAGCCCCCGCACCCTGAACGCCTGGGTGAAGG TGGTGGAGGAGAAGGCCTTCAGCCCCGAGGTGATCCCCATGTTCAGCGCCCTGAGCGAGGGCGCCACCCC CCAGGACCTGAACACGATGTTGAACACCGTGGGCGGCCACCAGGCCGCCATGCAGATGCTGAAGGAGACC ATCAACGAGGAGGCCGCCGAGTGGGACCGCGTGCACCCCGTGCACGCCGGCCCCATCGCCCCCGGCCAGA TGCGCGAGCCCCGCGGCAGCGACATCGCCGGCACCACCAGCACCCTGCAGGAGCAGATCGGCTGGATGAC CAACAACCCCCCCATCCCCGTGGGCGAGATCTACAAGCGGTGGATCATCCTGGGCCTGAACAAGATCGTG CGGATGTACAGCCCCACCAGCATCCTGGACATCCGCCAGGGCCCCAAGGAGCCCTTCCGCGACTACGTGG ACCGCTTCTACAAGACCCTGCGCGCTGAGCAGGCCAGCCAGGACGTGAAGAACTGGATGACCGAGACCCT GCTGGTGCAGAACGCCAACCCCGACTGCAAGACCATCCTGAAGGCTCTCGGCCCCGCGGCCACCCTGGAG GAGATGATGACCGCCTGCCAGGGCGTGGGCGGCCCCGGCCACAAGGCCCGCGTGCTGGCCGAGGCGATGA GCCAGGTGACGAACCCGGCGACCATCATGATGCAGCGCGGCAACTTCCGCAACCAGCGGAAGACCGTCAA GTGCTTCAACTGCGGCAAGGAGGGCCACACCGCCAGGAACTGCCGCGCCCCCCGCAAGAAGGGCTGCTGG CGCTGCGGCCGCGAGGGCCACCAGATGAAGGACTGCACCGAGCGCCAGGCCAACTTCCTGGGCAAGATCT GGCCCAGCTACAAGGGCCGCCCCGGCAACTTCCTGCAGAGCCGCCCCGAGCCCACCGCCCCCCCCGAGGA GAGCTTCCGCTTCGGCGAGGAGAAGACCACCCCCAGCCAGAAGCAGGAGCCCATCGACAAGGAGCTGTAC CCCCTGACCAGCCTGCGCAGCCTGTTCGGCAACGACCCCAGCAGCCAGGGATCCAAGGGCGAGGAGGATA ACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT CGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAG GGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGA AGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGAT GAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTAC AAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGG AGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCT GAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCC GGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGT ACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAACTCGAGTCTAGAGG GCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCC CCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCAT CGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTG GGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGG GGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCA GCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCAC GTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGG CACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTT TTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAA CCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAG CTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCC AGGCTCCCCAGGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTC CCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCC CTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTT TTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTT TGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACGTG TTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGC CAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGAC CGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGT TCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGA CGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACC GAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCG TGGCCGAGGAGCAGGACTGACACGTGCTACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGG CTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTC GCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAA ATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTG TATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT CCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGA GCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA TTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACT GACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCA AGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCT TTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCAC GAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTG AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGG ATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTT GGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCAT AGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCA ATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCG AGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGT AAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCG TCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGT GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACT CATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGT GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATAC GGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCA GCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAA TAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGG TTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACA TTTCCCCGAAAAGTGCCACCTGACGTC

Biovector

¥7930

2017-02-22

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pHIV-Luc-ZsGreen慢病毒双荧光表达载体质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pHIV-Luc-ZsGreen Plasmid 39196 -BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心描述： pHIV-Luc-ZsGreen慢病毒双荧光表达载体质粒，萤火虫酶荧光和ZsGreen绿色荧光通过IRES双顺反子元件连接双表达 Vector backbonepHIV-Zsgreen (Search Vector Database) Total vector size (bp)9312 Modifications to backboneThis plasmid was generated by cloning Luc2P (firefly luciferase) into the EcoRI and XbaI sites of pHIV-ZsGreen (Addgene Plasmid 18121). Vector typeMammalian Expression, Lentiviral GROWTH IN BACTERIA Bacterial Resistance(s)Ampicillin Growth Temperature37°C Growth Strain(s)Stbl3 Copy numberHigh Copy GENE/INSERT 1 Gene/Insert nameFirefly Luciferase Luc2P Insert Size (bp)1600 PromoterEF1-alpha CLONING INFORMATION FOR GENE/INSERT 1 Cloning methodRestriction Enzyme 5′ cloning siteEcoRI (not destroyed) 3′ cloning siteXbaI (not destroyed) 5′ sequencing primerEF1a Fwd Primer (Common Sequencing Primers) GENE/INSERT 2 Gene/Insert nameZsGreen Insert Size (bp)700 PromoterEF1-alpha CLONING INFORMATION FOR GENE/INSERT 2 Cloning methodRestriction Enzyme 5′ cloning siteMscI (not destroyed) 3′ cloning siteClaI (not destroyed) 5′ sequencing primern/a 3′ sequencing primerpBluescriptKS Primer pHIV-Luc-ZsGreen慢病毒双荧光表达载体质粒，萤火虫酶荧光和ZsGreen绿色荧光通过IRES双顺反子元件连接双表达.png >Full plasmid sequence gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata gttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagct acaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttc gcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggg gtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctga ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgacc ttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtttt ggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgt caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtct ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct cagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaaggga aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcg actggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagt attaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttaga tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaag ctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgcgctga tcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaa aattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtg ggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgc tgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattga ggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtg gaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactg ctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatgga gtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaa gaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaa attggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgc tgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaacc ccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattc gattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaatttta aaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacataca aactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagat ccagtttggttagtaccgggcccgctctagccgtgaggctccggtgcccgtcagtgggcagagcgcacat cgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcgg ggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatata agtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgt gtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggct gcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggt ggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgc gacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttttt ggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgc ggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgcc gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcac ccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgcc gtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttat gcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattct ccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttt ttttcttccatttcaggtgtcgtgagcggccgctgaactgAATTCATGgaagatgccaaaaacattaaga agggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcg ctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgag tacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcg tggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgt ggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtc gtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaaga tcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccattt gccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatc atgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgat tcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgcc atttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatg taccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgc ccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagat cgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggc atccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagc ctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacact gggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaacccc gaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgagg acgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagc cgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgac gatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcg tggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggt gcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggc ggcaagatcgccgtgtaataattctagagtacccgggctaggatccgcccctctccctccccccccccta acgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatatt gccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctt tcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttctt gaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctg cggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttgg atagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaagg taccccattgtatgggatctgatctggggcctcggtAcacatgctttacatgtgtttagtcgaggttaaa aaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatatggccac aaccatggcccagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatggagggctgcgtg gacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaagggcaagcaggccatcaacc tgtgcgtggtggagggcggccccttgcccttcgccgaggacatcttgtccgccgccttcatgtacggcaa ccgcgtgttcaccgagtacccccaggacatcgtcgactacttcaagaactcctgccccgccggctacacc tgggaccgctccttcctgttcgaggacggcgccgtgtgcatctgcaacgccgacatcaccgtgagcgtgg aggagaactgcatgtaccacgagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaa gaagatgaccgacaactgggagccctcctgcgagaagatcatccccgtgcccaagcagggcatcttgaag ggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctgccagttcgacaccgtgtaca aggccaagtccgtgccccgcaagatgcccgactggcacttcatccagcacaagctgacccgcgaggaccg cagcgacgccaagaaccagaagtggcacctgaccgagcacgccatcgcctccggctccgccttgccctaa ggatcgcatcgataccgtcgacctcgatcgagacctagaaaaacatggagcaatcacaagtagcaataca gcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacac ctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggg gggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaa ggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggt gctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgtt acaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgc ctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatct gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgct tcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtg tggaaaatctctagcagcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgc tggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcg aaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcca ctggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagt ggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagat ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttac caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcc ccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga cccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag ttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggc ttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtt agctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaa gtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcg ccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttacttt caccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgg aaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatga gcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagt gccacctgac

Biovector

¥9850

2017-02-22

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pDL278革兰氏阳性菌穿梭载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

PDL278革兰氏阳性菌穿梭载体 -BIOVECTOR NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 pDL278.png BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 PURPOSE (Empty Backbone) E. Coli/Gram-positive Bacterial Shuttle Vector Vector backbonepDL276 (Search Vector Database) Backbone manufacturerAddgene plasmid 46881 Backbone size (bp)6733 Modifications to backboneKanamycin resistance-encoding aphA3 gene and pVA380-1 ORF-1 of pDL276 replaced with spectinomycin resistance-encoding aad9 gene and pVA380-1 ORF-1 of pDL602 (see associated article for detailed construction information). Vector typeBacterial Expression ; E. coli/Gram-positive Bacterial Shuttle Vector GROWTH IN BACTERIA Bacterial Resistance(s)Spectinomycin Growth Temperature37°C Growth Strain(s)DH5alpha Growth instructionsFor E. coli, grow in Luria broth (LB) agar containing 50μg/mL Spectinomycin. For E. faecalis, select at 500μg/mL in culture and 1000μg/mL on plates.

Biovector

¥19300

2017-02-22

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pEarleyGate 104植物黄色荧光融合过表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pEarleyGate 104植物黄色荧光融合过表达载体gateway载体 -BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 pEarleyGate 104 allows for rapid recombinational cloning of cDNAs from Gateway entry vectors to produce Y-terminal fusions of encoded proteins in-frame with YFP. This vector is useful for cell localization studies and uses an enhanced CaMV 35S promoter to drive expression.

Biovector

¥19300

2017-02-22

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pEarleyGate 101，C-YFPHA植物黄色荧光融合过表达载体gateway载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pEarleyGate 101，C-YFPHA植物黄色荧光融合过表达载体gateway载体描述： C端带有YFP和HA标签，用于蛋白定位表达研究。 Name pEarleyGate101 Type plasmid Description pEarleyGate 101 allows for rapid recombinational cloning of cDNAs from Gateway entry vectors to produce C-terminal fusions of encoded proteins in-frame with YFP HA. This vector is useful for cell localization studies and uses an enhanced CaMV 35S promoter to drive expression Host Strain Genus Species Strain Name Escherichia coli DB3.1 Promoter:Reporter Names CaMV: Promoter:Reporter Information 35S: YFP-HA Selection kanamycin in bacteria BASTA in plants Images

Biovector

¥9850

2017-02-22

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pYES-DEST52酵母表达Gateway载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pYES-DEST52酵母表达Gateway载体描述：Amp抗性，URA3酵母筛选标记，PGAL1启动子，CYC1 polyA终止子，2u酵母复制起始位点。MAP图谱：BioVec...

Biovector

¥7930

2017-02-22

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pNIC28-Bsa4蔗糖致死性原核表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pNIC28-Bsa4蔗糖致死性原核原核表达载体描述：带有His和TEV标签，SacB蔗糖致死基因，Kan抗性，T7启动子及终止子，pET28a载体骨架。MAP图谱...

Biovector

¥9830

2017-02-22

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pKan-CMV-SB100X睡美人转座子表达质粒Sleeping beauty SB100X transposase

pKan-CMV-SB100X睡美人转座子表达质粒描述：CMV启动子表达Sleeping beauty SB100X transposase, Kan抗性。Map图谱：BioVector NTCC质...

Biovector

¥58920

2017-02-22

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pSBSO睡美人转座子系统载体Sleeping Beauty Transposon-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pSBSO睡美人转座子系统载体描述：目的基因前面带有hEF-1alpha启动子，左右两侧携带逆向重复序列Inverted Repeats, 可以被Sleeping Beauty transposon睡美人转座子特意识别结合，将目的基因整合到基因组中，配套载体pKan-CMV-SB11, pCMV-SB11, pCMV-SB100X，pCMVbeta-SleepingBeauty11，pCMVbeta-SleepingBeauty10等。货号BioVector504068 pSBSO 货号BioVector504069 pKan-CMV-SB11 货号BioVector504070 pCMV-SB100X 货号BioVector504071 pCMVbeta-SleepingBeauty11 货号BioVector504072 pCMVbeta-SleepingBeauty10 Map图谱： pSBSO睡美人转座子系统载体，目的基因前面带有hEF-1alpha启动子，左右两侧携带逆向重复序列Inverted Repeats, 可以被Sleeping Beauty transposon睡美人转座子特意识别结合，将目的基因整合到基因组中，配套载体pKan-CMV-SB11, pCMV-SB11, pCMV-SB100X等。BioVector NTCC质粒载体菌种细胞基因保藏中心.png BioVector NTCC质粒载体菌种细胞基因保藏中心

Biovector

¥57920

2017-02-22

在线浏览

pKan-CMV-SB11睡美人转座子表达质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pKan-CMV-SB11睡美人转座子表达质粒描述：CMV启动子表达sleeping beauty SB11 transposase, Kan抗性。Map图谱：BioVector NTCC质粒载...

Biovector

¥19300

2017-02-22

在线浏览

pCMV-SB11睡美人转座子系统表达质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pCMV-SB11睡美人转座子系统表达质粒表达Sleeping Beauty transposon SB11Map图谱：