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MaV203 yeast strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥29862
  • 货  号:MaV203 yeast strain酵母菌株
  • 产  地:北京
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MaV203 yeast strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with most GAL4-based two-hybrid systems. This strain has been constructed with three GAL4-inducible reporter genes for identification of interacting fusion proteins: GAL1::lacZ, HIS3UASGAL1::HIS3 and the counterselectable SPAL10UASGAL1::URA3.1–3 MaV203 is also auxotrophic for leucine (leu2) and tryptophan (trp1-901), allowing for the selection of yeast transformed with GAL4 DNA binding domain (DB) vectors and GAL4 activation domain (AD) vectors (e.g., pDBLeu and pPC86 from the ProQuestTM Two-Hybrid System). MaV203 also contains the recessive resistance alleles cyh2R and canR which are useful for plasmid shuffling.
Genotype:MAT; leu2-3,112; trp1-901; his3200; ade2-101; cyh2R; can1R; gal4; gal80; GAL1::lacZ; HIS3UASGAL1::HIS3@LYS2; SPAL10 UASGAL1::URA3.
Note: While the genotype of MaV203 is ade2–, the strain remains white upon starvation for adenine or amino acids, but retains an ade2 deficiency.
【Supplier来源】BioVector NTCC Inc.
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