枯草芽孢杆菌非必须单基因敲除菌株库 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥98652
- 货 号:枯草芽孢杆菌非必须单基因敲除菌株库
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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枯草芽孢杆菌非必须单基因敲除菌株库 BioVector NTCC质粒载体菌种细胞基因保藏中心
枯草芽孢杆菌非必须单基因敲除菌株库,枯草芽孢杆菌基础基因敲低菌株库,3984种非必须基因敲除替代为Kan卡那霉素抗性基因,利用CRISPRi干扰技术敲低289种必须基因,木糖诱导表达dCas9及单一组成型表达sgRNA靶向必须基因5'端。
Bacillus subtilis is a key Gram positive model bacterium used to study diverse research topics such as chromosome replication, competence, or sporulation. Despite being well-studied and having a fully sequenced genome, there are still many unknown or poorly characterized genes. To facilitate better understanding of individual B. subtilis genes, the Gross lab at UCSF has constructed 2 ordered libraries:
Kit 1000000115
B. subtilis Single Gene Deletion Library--Kanamycin is an arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
This kit will be sent as bacterial glycerol stocks in a 384-well plate format.
Kit 1000000116
B. subtilis CRISPRi Essential Gene Knockdown Library is an arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
This kit will be sent as bacterial glycerol stocks in a 384-well plate format.
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
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