ZYCY10P3S2T minicircle载体生产菌株/感受态细胞BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Biovector691470
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minicircle感受态细胞ZYCY10P3S2T BioVector NTCC质粒载体菌种细胞基因保藏中心
Cat# Biovector691470
E.coli ZYCY10P3S2T
E.coli. 1Vial.
BioVector's ZYCY10P3S2T Minicircle Production Strain, Simplifying minicircle production
Optimized for minicircle production, the BioVector's ZYCY10P3S2T Minicircle Production Strain comes as
competent cells, so you can directly transform ligation reactions or plasmids. The competent cells are
prepared and tested using a modification of the procedure developed by Kay, et al., and are suitable
for cloning and production of minicircle-based plasmids.
The BioVector's ZYCY10P3S2T E.coli Minicircle Production Strain is derived from the BioVector's BW27783 E. coli strain
that stably expresses a set of inducible minicircle-assembly enzymes, PhiC31 integrase, and I-SceI
homing endonuclease. This bacterial strain enables production and purification of minicircles in a
time frame and quantity similar to that of routine plasmid DNA preparation.
Please note that other competent E. coli strains cannot be substituted for minicircle production as
they do not have the appropriate genomic modifications to produce minicircles.
About Minicircle Technology
Episomal expression sustained over weeks
Foreign DNA-free
More efficient transfections from small plasmid size
Unlimited insert size
Optimized coli minicircle production strain
Works in vitro and in vivo
When you want sustained transgene expression without introducing any foreign DNA—such as for
model animal and gene therapy development—Minicircle Technology is a great gene expression
option. Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral,
episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes
with a variety of promoter and reporter combinations. Their small size facilitates more efficient
transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not
replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue
for months in non-dividing cells.
Figure 1. Generating minicircle DNA from the Parental Minicircle Plasmid.
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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