KE-37 cell line人急性淋巴细胞白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KE-37 cell line人急性淋巴细胞白血病细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: KE-37 DSMZ no.: ACC 46 Species: human (Homo sapiens) Cell type: T cell leukemia Origin: established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979 Reference(s): 14473 Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology: single, round cells in suspension Medium: 90-92.5% RPMI 1640 + 7.5-10% h.i. FBS Subculture: split saturated culture 1:2 to 1:3 every second day; maintain at about 1-1.5 x 106 cells/ml; seed out at ca. 1-2 x 106 cells/ml; initially after thawing, cells should be cultured with 20% FBS for the first 1-2 weeks; maximum density at ca. 3 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 40 hours Harvest: cell harvest of ca. 2 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: CD2 +, cyCD3 +, smCD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -;image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH Cytogenetics: human flat-moded hypotetraploid karyotype - 91(86-92)<4n>XXYY, +8, -14, -14, t(7;12)(q32-33;p12-13)x2, der(8)t(8;14)(q24;q11)x4, der(14)t(8;14)(q24;q11)x2 - resembles published karyotype - carries 4 copies of der(8)t(8;14) associated with T-ALL - normal ch 14 present Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, MLV -, SMRV -
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