Bacteroides plebeius strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心

Bacteroides plebeius strain菌株菌种 BioVector NTCC质粒载体菌种细胞基因保藏中心
Bacteroides plebeius Kitahara et al. 2005
Cat No.:
17135, Type strain
Strain designation:
M12
Isolated from:
human feces
Country:
Japan
Date of sampling:
02-2004
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
Genbank accession numbers:
16S rRNA gene: AB200217
whole genome shotgun sequence: ABQC00000000
Cultivation conditions:
Medium CHOPPED MEAT MEDIUM WITH CARBOHYDRATES , anaerobic, 37°C
Medium/media recipe(s) required for preparing 110:
- Medium 78
or
Medium 693 , anaerobic, 37°C
Incubation time: 3-7 days
Please follow special instructions: 'Cultivation of Anaerobes'
CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
Ground beef (fat free) 500.0 g
Distilled water 1000.0 ml
NaOH 1 N 25.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix
meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature,
skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate
add water to a final volume of 1000 ml, and then add:
Casitone 30.0 g
Yeast extract 5.0 g
K2HPO4 5.0 g
Na-resazurin solution (0.1% w/v) 0.5 ml
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D-Glucose 4.0 g
Cellobiose 1.0 g
Maltose 1.0 g
Starch (soluble) 1.0 g
To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l Lcysteine
hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml
medium into Hungate-type tubes (for strains demanding meat particles put those first
into th
min. For agar slants use 15.0 g agar per 1000.0 ml medium.
In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or
Vitamin K3 is necessary. Add to 1000.0 ml of medium after autoclaving:
Haemin solution (see below) 10.00 ml
Vitamin K1 or Vitamin K3 solution (see below) 10.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and
filter sterilize. Store refrigerated.
Vitamin K1 solution:
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.
Vitamin K3 solution:
Dissolve 5 mg/ml of vitamin K3 in 95% ethanol,
Summary and
additional information:
<- Maki Kitahara, M12. Human feces; Japan. Type strain.Taxonomy/description (9969). Sequence accession no. 16S rRNA gene: AB200217
(9969), whole genome shotgun sequence: ABQC00000000. (Medium 110, 37°C, anaerobic or Medium 693, 37°C, anaerobic).
Risk group:
1
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