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Fluorescent Proteins: Subcellular Localization plasmids vectors亚细胞定位质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:Fluorescent Proteins: Subcellular Localization
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Fluorescent Proteins: Subcellular Localization plasmids vectors亚细胞定位质粒载体

Background The subcellular localization of a protein is often tied to its function, so it's important to determine where your protein of interest resides. There are a variety of methods for determining subcellular localization. One of the most common is to use fluorescence microscopy to compare the localization of your protein of interest with known markers.
BioVector has assembled a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest with a fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess whether your protein is targeted to the same structures as these well-characterized proteins.
General Structures The cell contains a variety of organelles and subcellular structures each with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria produces the energy cells required for chemical reactions. Labeling these structures can be quite useful for identifying the localization or possible function of a protein of interest. Browse or search the table below to find markers of general cellular organelles and structures.
Showing 1 to 56 of 56 entries Show entries Search table: Previous1Next Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI CAV1-mCherry Caveolae Cav1 mCherry Helenius pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez H2B-mCherry Chromatin H2B mCherry Benezra H2B-GFP Chromatin H2B GFP Wahl GFP-HP1gamma Chromatin HP1 gamma GFP Tom Misteli pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich PGK-H2BeGFP Chromatin H2B EGFP Mark Mercola PGK-H2BmCherry Chromatin H2B mCherry Mark Mercola pHIV-H2BmRFP Chromatin H2B mRFP Bryan Welm, Zena Werb LV-GFP Chromatin H2B GFP Elaine Fuchs pH2B-miRFP703 Chromatin H2B miRFP703 Verkhusha pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez cfSGFP2-N Extracellular milieu cfSGFP2-N EGFP Wada pCAG-mGFP Membrane palmitoylation sequence from GAP43 EGFP Cepko pPalmitoyl-mTurquoise2 Membrane palmitoylation sequence from p63 mTurquoise2 Gadella Lck-GFP Membrane palmitoylation sequence from Lck EGFP Green pQC membrane TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko FUmGW Membrane Palmitoylation GFP Connie Cepko pmTurquoise2-Mito Mitochondria COX8A(1-29) mTurquoise2 Gadella mCherry-Mito-7 Mitochondria Mitochondrialtargeting sequence(COX8A) mCherry* Davidson mito-BFP Mitochondria mitochondrial targeting signal (COX4) TagBFP Voeltz pLV-mitoGFP Mitochondria COX8 GFP Pantelis Tsoulfas mCherry-Parkin Mitochondria Park2 mCherry Richard Youle pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha pABCb10-GFP Mitochondria ABCb10 GFP Shirihai pMito-miRFP703 Mitochondria COX8A miRFP703 Verkhusha pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez pCMV-mGold-Mito-N-7 Mitochondria Mito mGold Francois St-Pierre GFP-Mff Mitochondria-Outer Membrane Mff acGFP1 Voeltz mCherry-TOMM20-N-10 Mitochondria-Outer Membrane TOMM20 mCherry* Davidson pmTurquoise2-NES Non-nucleus Nuclear Export Sequence mTurquoise2 Gadella pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Gadella pmScarlet-i_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-I Gadella pmScarlet-H_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet-H Gadella mCherry-LaminB1-10 Nuclear Envelope LaminB1 mCherry* Davidson mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Davidson pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Huang pEBFP2-Nuc Nucleus NLS EBFP2 Campbell pmTurquoise2-H2A Nucleus H2A mTurquoise2 Gadella pQC NLS mCherry IX Nucleus NLS mCherry Cepko pEGFP-SF2 Nucleus SF2 EGFP Tom Misteli pmKalama1-Nuc Nucleus NLS mKamala1 Campbell pmScarlet_H2A_C1 Nucleus H2A mScarlet Gadella pQC NLS YFP IX Nucleus NLS YFP Cepko pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Gadella pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez pmScarlet-H_H2A_C1 Nucleus H2A mScarlet-H Gadella pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-Pierre pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Dammeyer pmScarlet_peroxisome_C1 Peroxisome SRL mScarlet Gadella pmScarlet-I_peroxisome_C1 Peroxisome SRL mScarlet-I Gadella pmScarlet-H_peroxisome_C1 Peroxisome SRL mScarlet-H Gadella pmTurquoise2-Peroxi Peroxisomes Peroxisomal Targeting Sequence mTurquoise2 Gadella mCherry-Peroxisomes-2 Peroxisomes Peroximal Targeting Signal 1 (PTS1) mCherry* Davidson pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisomes SKL tripeptide, peroxisome transport signal dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.
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Cytoskeletal Elements The cytoskeleton is the cell’s internal framework that assists in sustaining structure and function. The cytoskeleton is primarily made up of microtubules, actin filaments, and intermediate filaments. These structures give the cell its shape, organize cellular organelles, and are necessary for movement and cell division. Browse or search the table below to find markers of cytoskeletal element.
Showing 1 to 44 of 44 entries Show entries Search table: Previous1Next Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI GFP-cortactin Actin Filaments Cortactin EGFP Anna Huttenlocher pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin 1 EGFP Carol Otey Cortactin-pmCherryC1 Actin Filaments Cortactin mCherry Christien Merrifield pLifeAct-mTurquoise2 Actin Filaments LifeAct mTurquoise2 Dorus Gadella pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella pEGFP-C1 F-tractin-EGFP Actin Filaments F-tractin EGFP Dyche Mullins mCherry-Lifeact-7 Actin Filaments LifeAct mCherry* Michael Davidson mEGFP-Lifeact-7 Actin Filaments LifeAct EGFP Michael Davidson eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer pDEST/LIfeAct-mCherry-N1 Actin Filaments LifeAct mCherry Robin Shaw pCAG-mGFP-Actin Actin Filaments beta-actin EGFP Ryohei Yasuda pPAmCherry-b-actin Actin Filaments beta-actin PAmCherry1 Vladislav Verkhusha GFP-UtrCH Actin Filaments utrophin (aa# 1-261) GFP William Bement mCherry-UtrCH Actin Filaments Utrophin mCherry William Bement pLifeAct_mScarlet-i_N1 Actin Filaments LifeAct mScarlet-I Dorus Gadella pLifeAct-miRFP703 Actin Filaments LifeAct miRFP703 Vladislav Verkhusha pLifeAct_mScarlet-H_N1 Actin Filaments LifeAct mScarlet-H Dorus Gadella pCMV-mGold-Actin-C-18 Actin Filaments Actin mGold Francois St-Pierre GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson pKeratin-miRFP703 Intermediate Filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha pEGFP-mNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown pEGFP-rNFM Intermediate Filaments (neuronal) Nefm EGFP Anthony Brown pVimentin-miRFP703 Intermediate Filaments (vimentin) vimentin miRFP703 Vladislav Verkhusha pmTurquoise2-Tubulin Microtubules alpha-tubulin mTurquoise2 Dorus Gadella mCh-alpha-tubulin Microtubules alpha-tubulin mCherry Gia Voeltz pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth EMTB-3XGFP Microtubules ensconsin GFP William Bement EMTB-mCherry Microtubules ensconsin mCherry William Bement pmScarlet_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet Dorus Gadella mCherry-Tubulin-C-18 Microtubules alpha-tubulin mCherry* Michael Davidson pPAmCherry-a-tubulin Microtubules alpha-tubulin PAmCherry Vladislav Verkhusha pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus Gadella pmScarlet-H_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-H Dorus Gadella pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw pmiRFP703-Tubulin Microtubules alpha-tubulin miRFP703 Vladislav Verkhusha pEB3-miRFP703 Microtubules EB3 miRFP703 Vladislav Verkhusha GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.
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Cell Junctions Cell junctions are responsible for forming connections and adhesions between animal cells. They are important for holding cell structures together and reducing cell stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers for cellular junctions.
Showing 1 to 9 of 9 entries Show entries Search table: Previous1Next Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI E-cadherin-GFP Adherens junctions E-cadherin GFP Stow Paxillin-pEGFP Focal adhesions paxillin GFP Horwitz pPaxillin-PSmOrange Focal adhesions paxillin PSmOrange Verkhusha mEmerald-Paxillin-22 Focal Adhesions paxillin mEmerald* Davidson pZyxin-miRFP703 Focal adhesions Zyxin miRFP703 Verkhusha pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Shaw pDEST-mCherry-GJA1-20k-N1 Gap Junctions Connexin 43 / GJA1-20k mCherry Shaw mCherry-Cx43-7 Gap Junctions Connexin 43 / GJA1-20k mCherry* Davidson mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Davidson *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.
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Secretory Pathway The secretory pathway is named for its role in secreting proteins from the cell to the extracellular environment. Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in vesicles to be packaged and shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding or post-translational modification to function properly. Browse or search the table below to find markers of secretory pathway components.
Showing 1 to 48 of 48 entries Show entries Search table: Previous1Next Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI pEGFP-LC3 (human) Autophagosome LC3 EGFP Finkel pCMB-AUTr Autophagosome LC3(ATG8) mCherry Harrison mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Helenius Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Zhuang mEmerald-Clathrin-15 Clathrin-coated vesicles Clathrin mEmerald* Davidson pmiRFP703-Clathrin Clathrin-coated vesicles Clathrin miRFP703 Verkhusha DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Pagano mRFP-Rab5 Early endosomes RAB5A mRFP Helenius mCh-Rab5 Early endosomes Rab5 mCherry Voeltz BFP-Rab5 Early endosomes Rab5 BFP Voeltz GFP-Rab5B Early endosomes Rab5B AcGFP Voeltz GFP-Rab4B Early endosomes Rab4B AcGFP Voeltz pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP Johnson pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP Johnson pEF.myc.ER-E2-Crimson Endoplasmic Reticulum ER retention signal E2-Crimson Glick pmTurquoise2-ER Endoplasmic Reticulum ER retention signal mTurquoise2 Gadella mCh-Sec61 beta Endoplasmic Reticulum Sec61 mCherry Voeltz BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Voeltz BFP-KDEL Endoplasmic Reticulum BiP ER signal sequence BFP Voeltz Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP Voeltz ERmoxGFP Endoplasmic Reticulum ER retention signal moxGFP Snapp ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Snapp pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic reticulum (ER) KDEL dL5 FAP (fluorogen activating protein); mCerulean3 fusion Bruchez pCytERM_mScarlet-i_N1 ER CytERM mScarlet-I Gadella pCytERM_mScarlet-H_N1 ER CytERM mScarlet-H Gadella pCytERM_mScarlet_N1 ER CytERM mScarlet Gadella pmTurquoise2-Golgi Golgi B4GALT1(1-61) mTurquoise2 Gadella GolgiCFP Golgi eNOS(1-33) CFP Newton GalToxBFP Golgi GalT signal anchor transmembrane domain oxBFP Snapp pmScarlet-i_Giantin_C1 Golgi apparatus Giantin mScarlet-I Gadella pmScarlet-H_Giantin_C1 Golgi apparatus Giantin mScarlet-H Gadella pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet Gadella GFP-rab7 WT Late endosomes RAB7 GFP Pagano DsRed-rab7 WT Late endosomes RAB7 DsRed Pagano mCh-Rab7A Late endosomes Rab7a mCherry Voeltz GFP-Rab7A Late endosomes Rab7a AcGFP Voeltz pTag-RFP-C-h-Rab7-c-Myc Late endosomes Rab7 TagRFP Johnson pTag-BFP-C-h-Rab7-c-Myc Late endosomes Rab7 TagBFP Johnson Lamp1-YFP Lysosomes Lamp-1 YFP Mothes Lamp1-RFP Lysosomes Lamp-1 RFP Mothes mCherry-Lysosomes-20 Lysosomes LAMP1 mCherry* Davidson pLAMP1-miRFP703 Lysosomes LAMP1 miRFP703 Verkhusha GFP-rab11 WT Recycling endosomes RAB11A GFP Pagano DsRed-rab11 WT Recycling endosomes RAB11A DsRed Pagano pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP Johnson pTag-BFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagBFP Johnson pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP Johnson pTag-BFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagBFP Johnson *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection.

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