• Vector backbone
  LZRS
  (Search Vector Database)

• Backbone size w/o insert (bp)11100

• Vector type
  Retroviral
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl2
• Growth instructions
  STBL2
• Copy number
  Low Copy

GENE/INSERT

• Gene/Insert name
  HOTAIR
• Species
  H. sapiens (human)
• Insert Size (bp)
  2146
• Entrez Gene
  HOTAIR(a.k.a. HOXAS, HOXC-AS4, HOXC11-AS1, NCRNA00072)

CLONING INFORMATION

• Cloning methodGateway Cloning

• 5′ cloning siteattR (destroyed during cloning)

• 3′ cloning siteattR (destroyed during cloning)

• 5′ sequencing primerTGGATACACGCCGCCCACGTG

• 3′ sequencing primerATCGTCGACCACTGTGCTGG