Thermus thermophilus HB8极端嗜热菌模式菌株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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- 货 号:Thermus thermophilus HB8极端嗜热菌
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Thermus thermophilus (ex Oshima and Imahori 1974) Manaia et al. 1995
NTCC No.:
NTCC59-579, Type strain
Strain designation:
HB8
Other collection no.
or WDCM no.:
ATCC 27634
Isolated from:
hot spring
Geographic origin:
Japan
History:
<- T. Oshima, HB8 (formerly Flavobacterium thermophilum)
Genbank accession numbers:
16S rRNA gene: X07998
Cultivation conditions:
Medium 74 , 75°C
THERMUS THERMOPHILUS MEDIUM Yeast extract (BD Difco) 4.0 g Proteose peptone Nr. 3 (BD
Difco) 8.0 g NaCl 2.0 g Distilled water 1000.0 ml Adjust pH to 7.0. The original medium description
specifies polypeptone, which appears to be no longer available.
or
Medium 878 , 75°C
THERMUS 162 MEDIUM Yeast extract 1.00 g Tryptone 1.00 g Agar 28.00 g Nitrilotriacetic acid 100.00
mg CaSO4 x 2 H2O 40.00 mg MgCl2 x 6 H2O 200.00 mg 0.01 M Fe citrate 0.50 ml Trace element
solution (see below) 0.50 ml Phosphate buffer (see below) 100.00 ml Distilled water 900.00 ml C for 15
min.Adjust pH to 7.2 with NaOH. Autoclave at 121 Autoclave the phosphate buffer separately and then
add to the medium. Phosphate buffer: KH2PO4 5.44 g Na2HPO4 x 12 H2O 43.00 g Distilled water
1000.00 ml Adjust pH to 7.2 Trace element solution: H2SO4 0.50 ml MnSO4 x H2O 2.28 g ZnSO4 x 7
H2O 0.50 g H3BO3 0.50 g CuSO4 x 5 H2O 25.00 mg Na2MoO4 x 2 H2O 25.00 mg CoCl2 x 6 H2O
45.00 mg Distilled water 1000.00 ml
Summary and additional information:
<- T. Oshima, HB8 (formerly Flavobacterium thermophilum). Hot spring; Japan. Type strain. Taxonomy/description (655,
656, 2248, 6031, 6056, 6634). Produces restriction endonuclease TthHB8I (2358), thermostable enzymes (657, 658),
thermostable D-xylose isomerase (4701). Minimal medium (2248). Fatty acid composition (2249). Stringent control
(2329). (Medium 74 or 878, 75°C)
Risk group:
1
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