YPH499酿酒酵母菌株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

YPH499酿酒酵母菌株

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Description

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for

selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS

sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose

anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original

source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.

Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.

Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.