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2017-12-21
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35S-Luc
Biovector
大肠杆菌DH5A supE44& lacU 196(U80lacZ&M 15) recA endA
成团泛菌308R 抗利福霉素自发突变株, 从番茄上分离
pExSec1 分泌表达载体, 与蛋白A 的 SZZ 融合表达, 受 T7启动子严谨调控,Km
r
, Tc
Strains
Escherichia coli DH5α supE44 lacU169 (80lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [20]
Human Nanog Differentiation Reporter (pGreenZeo, plasmid)
Retrovirus kit for reprogramming (concentrated virus pool of Human Oct4, Sox2, Klf4, cMyc, 4X10^6 IFUs per construct per vial)
pGreenFire1-NF-kB (plasmid)+ EF1-Puro
pGreenFire1-RARE (plasmid)+ EF1-Neo
pGreenFire1-SMAD (plasmid)
pGreenFire1-SMAD (virus) + EF1-Neo
pBABE-EGFP-mCherry-LC3细胞自噬质粒载体
pMTL-cpb2
B. subtilis WH558 trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-lacZ Bertram et al., 2005
B. subtilis RAB101 trpC2 lacA::Pt17-tetR-lox66-aphAIII-lox71-tetR;lox72 amyE::Pxyl/tet-lacZ this study
pCrePA EmR, PpagA-cre thermosensitive, expression of cre in Bacillus Pomerantsev et al., 2006
pWH1411-TLKLTextd pWH1411BD derivative, tetR-lox66-aphAIII-lox71-tetR ‘lacA’ This study