Genome-CRISP™ 诱导型 Cas9 人类 AAVS1 safe harbor 基因敲入试剂盒 - Blast BioVector NTCC质粒载体菌种细胞基因保藏中心

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Genome-TALER™ Cas9 人类 AAVS1 safe harbor 基因敲入试剂盒 - Puro BioVector NTCC质粒载体菌种细胞基因保藏中心

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Genome-TALER™ Cas9 人类 AAVS1 safe harbor 基因敲入试剂盒 - Neo BioVector NTCC质粒载体菌种细胞基因保藏中心

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Genome-TALER™ 诱导型 Cas9 人类 AAVS1 safe harbor 基因敲入试剂盒 - Blast BioVector NTCC质粒载体菌种细胞基因保藏中心

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人类 AAVS1 ORF 敲入克隆 BioVector NTCC质粒载体菌种细胞基因保藏中心

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HOP-FLASH信号通路荧光报告质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

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TZM-bl cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

TZM-bl cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心Biohazardous: YesSpecial Characteristics: The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either β-gal or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran in the infection medium. The ß-gal and luciferase genes are also induced by HIV-2 infection.Cell Type: TZM-bl, previously designated JC53-bl (clone 13) is a HeLa cell line. The parental cell line (JC.53) stably expresses large amounts of CD4 and CCR5. The TZM-bl cell line was generated from JC.53 cells by introducing separate integrated copies of the luciferase and ß-galactosidase genes under control of the HIV-1 promoter. The TZM-bl cell line is highly sensitive to infection with diverse isolates of HIV-1.Freeze Medium: 50% FBS, 40% DMEM, 10% DMSO.Growth Characteristics: Adherent cell line. No special requirements for thawing and reestablishing the culture. Cells grow in single cell layer. Antibiotic selection is not required to maintain stable expression of the receptors and reporter genes.Propagation Medium: DMEM (90%), 10% FBS, 100 units of Penicillin and 0.1 mg/ml of Streptomycin.Sterility: Negative for bacteria, mycoplasma, and fungi.References: Platt EJ, Bilska M, Kozak SL, Kabat D, Montefiori DC. Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. J Virol.2009;83(16):8289-92. Takeuchi Y, McClure MO, Pizzato M. Identification of γ-retroviruses constitutively released from cell lines used for HIV research. J Virol 82(24):12585-8, 2008 Abstract Wei X, Decker JM, Liu H, Zhang Z, Arani RB, Kilby JM, Saag MS, Wu X, Shaw GM, Kappes JC. Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy. Antimicrob Agents Chemother 46:1896-1905, 2002. Derdeyn CA, Decker JM, Sfakianos JN, Wu X, O'Brien WA, Ratner L, Kappes JC, Shaw GM, Hunter E. Sensitivity of human immunodeficiency virus type 1 to the fusion inhibitor T-20 is modulated by coreceptor specificity defined by the V3 loop of gp120. J Virol74:8358-8367, 2000. Platt EJ, Wehrly K, Kuhmann SE, Chesebro B, Kabat D. Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1. J Virol 72:2855-2864, 1998.【Supplier来源】BioVector NTCC Inc.TEL：+86-010-53513060【Website网址】 http://www.biovector.net

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pQE80L-Kan原核组成型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pQE80L-Kan原核组成型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心pQE80L-Kan原核组成型表达载体，带有T5启动子，6xHis标签，Kan抗性。【Supplier来源】BioVector NTCC Inc.TEL：+86-010-53513060【Website网址】 http://www.biovector.net

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pSEM1集孢藻表达质粒载体Synechocystis expression vector BioVector NTCC质粒载体菌种细胞基因保藏中心

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pCox1 mgdE原核共表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

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