FAS,FASN,OA-519,MGC14367,MGC15706 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

FAS,FASN,OA-519,MGC14367,MGC15706蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063242,BC063242.1,BU173262,BU173262.1 [Species物种来源]: Homo sapiens 【 FAS,FASN,OA-519,MGC14367,MGC15706蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ FAS,FASN,OA-519,MGC14367,MGC15706 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 FAS,FASN,OA-519,MGC14367,MGC15706 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens FAS,FASN,OA-519,MGC14367,MGC15706 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 FAS,FASN,OA-519,MGC14367,MGC15706 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens FAS,FASN,OA-519,MGC14367,MGC15706 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 FAS,FASN,OA-519,MGC14367,MGC15706 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 FAS,FASN,OA-519,MGC14367,MGC15706 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063275,BC063275.1,CB960781,CB960781.1 [Species物种来源]: Homo sapiens 【 Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 Q99,AGO1,EIF2C,EIF2C1,GERP95,DKFZP686M13167 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

ETK,HEK,ETK1,HEK4,EPHA3,TYRO4蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063282,BC063282.1,CB990159,CB990159.1 [Species物种来源]: Homo sapiens 【 ETK,HEK,ETK1,HEK4,EPHA3,TYRO4蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ETK,HEK,ETK1,HEK4,EPHA3,TYRO4 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063284,BC063284.1,BI597477,BI597477.1 [Species物种来源]: Homo sapiens 【 CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CASD1,C7ORF12,FLJ21213,FLJ21879,FLJ41901,NBLA04196 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

NOVEL,ZBTB21,ZNF295,DKFZP781N1974蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063290,BC063290.1,CB962022,CB962022.1 [Species物种来源]: Homo sapiens 【 NOVEL,ZBTB21,ZNF295,DKFZP781N1974蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NOVEL,ZBTB21,ZNF295,DKFZP781N1974 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens NOVEL,ZBTB21,ZNF295,DKFZP781N1974 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NOVEL,ZBTB21,ZNF295,DKFZP781N1974 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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ACDP4,CNNM4,KIAA1592 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

ACDP4,CNNM4,KIAA1592蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063295,BC063295.1,CB960058,CB960058.1 [Species物种来源]: Homo sapiens 【 ACDP4,CNNM4,KIAA1592蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ACDP4,CNNM4,KIAA1592 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ACDP4,CNNM4,KIAA1592 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ACDP4,CNNM4,KIAA1592 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ACDP4,CNNM4,KIAA1592 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens ACDP4,CNNM4,KIAA1592 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ACDP4,CNNM4,KIAA1592 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ACDP4,CNNM4,KIAA1592 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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RNF44,KIAA1100 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

RNF44,KIAA1100蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063297,BC063297.1,CB997744,CB997744.1 [Species物种来源]: Homo sapiens 【 RNF44,KIAA1100蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ RNF44,KIAA1100 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 RNF44,KIAA1100 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens RNF44,KIAA1100 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 RNF44,KIAA1100 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens RNF44,KIAA1100 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 RNF44,KIAA1100 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 RNF44,KIAA1100 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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NPC,NPC1 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

NPC,NPC1蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063302,BC063302.1,CB987975,CB987975.1 [Species物种来源]: Homo sapiens 【 NPC,NPC1蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ NPC,NPC1 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 NPC,NPC1 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens NPC,NPC1 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 NPC,NPC1 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens NPC,NPC1 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 NPC,NPC1 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 NPC,NPC1 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063304,BC063304.1,CD110228,CD110228.1 [Species物种来源]: Homo sapiens 【 ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 ANPA,NPR1,NPRA,ANPRA,GUC2A,GUCY2A Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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CUL5,VACM1,VACM-1 Protein & Antibody, Cell Lysate蛋白/抗体/细胞裂解液-BioVector NTCC基因蛋白抗体保藏中心

CUL5,VACM1,VACM-1蛋白/抗体/细胞裂解液Protein and Antibody， Cell Lysate [NCBI Accession Number基因编号]: BC063306,BC063306.1,CB996454,CB996454.1 [Species物种来源]: Homo sapiens 【 CUL5,VACM1,VACM-1蛋白产品信息】 [Protein Construction蛋白构建]: A DNA sequence encoding the Target Protein was expressed. [Tag标签]: 1.Fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus. OR 2.Polyhistidine tag OR 3.Fc region of human IgG1 OR 4.Fc region of rabbit IgG [Species分子种属]: Homo sapiens [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect Cells/ E.coli [Shipping运输说明]: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. [ CUL5,VACM1,VACM-1 Protein QC Testing蛋白质控检测] Purity纯度: > 97 % as determined by SDS-PAGE Endotoxin内毒素: < 1.0 EU per μg of the protein as determined by the LAL method Stability稳定性: Samples are stable for up to twelve months from date of receipt at -70℃ Formulation缓冲液: Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. [Protein Usage Guide蛋白使用指导] [Storage储存方法]: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. [Reconstitution复溶条件]: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. 【 CUL5,VACM1,VACM-1 Antibody Product Information抗体产品信息】 [Immunogen免疫原]: Recombinant Homo sapiens CUL5,VACM1,VACM-1 protein [Ig Type Ig类型]: Mouse/Rabbit IgG, Mouse IgG1, IgM, IgGM, IgG2a, IgG2b [Formulation缓冲液]: 0.2 μm filtered solution in PBS [Preparation抗体制备方法]: For Mouse MAb: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Target Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. For Rabbit PAb, Antigen Affinity Purified: Produced in rabbits immunized with purified, recombinant Target Protein. Target Protein specific IgG was purified by Target Protein affinity chromatography. For Rabbit PAb: Produced in rabbits immunized with purified, recombinant Target Protein. Total IgG was purified by Protein A affinity chromatography. 【 CUL5,VACM1,VACM-1 Antibody Usage Guide抗体使用指导】 [Specificity特异性]: Homo sapiens CUL5,VACM1,VACM-1 [Application推荐应用]: ICC/IF, IF, FCM FCM: 0.5-2 μg/TestICC/IF: 10-25 μg/mL ELISA ELISA: 0.5-1 μg/mL WB WB: 5-10 μg/ml IP IP: 1-4 μg/mg of lysate IHC-P IHC-P: 0.1-2 μg/mL [Storage储存方法]: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. 【 CUL5,VACM1,VACM-1 Transfected / Overexpression Cell Lysate Product Information细胞裂解液产品信息】 [Expressed Host表达宿主]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells [Product Description产品介绍]: Human Cells/ Baculovirus-Insect cells/ CHO Stable Cells lysate that Target Protein transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). 序列信息: For Human Cells/CHO Stable Cells expression: A DNA sequence encoding the Target Protein was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. For Baculovirus-Insect cells expression:A DNA sequence encoding the Target Protein was fused with (the N-terminal polyhistidine-tagged GST tag at the N-terminus)/ (a polyhistidine tag at the C-terminus). 【 CUL5,VACM1,VACM-1 Transfected / Overexpression Cell Lysate Usage Guide细胞裂解液使用指导】 [Preparation Method制备方法]: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. [Lysis Buffer裂解缓冲液]: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. [Quality Control Testing质控]: 12.5% SDS-PAGE Stained with Coomassie Blue after protein purification. [Stability稳定性]: Samples are stable for up to twelve months from date of receipt. [Recommend Usage使用建议]: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃. [Storage Buffer储存缓冲液]: 1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer). [Storage Instruction储存方法]: Store at 4℃. After re-dissolution, aliquot and store at -80℃. [Application notes应用提示]: Western blot (WB): Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

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