eHAP cells细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

eHAP cells细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心 Quality-controlled fully-haploid engineered HAP1 (eHAP) wild-type cells
Parental Cell Line
HAP1
Adherent Suspension
Adherent
General Information
Organism
Homo sapiens
Storage
LN vapor (less than -130°C)
Cell Culture
Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Growth Properties
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.

HAP1 are a fibroblast-like cell line used for biomedical research. Like all cancer cell lines, it is immortal and can divide indefinitely. They have been derived from the KBM-7 cell line through cellular reprogramming. A unique aspect of the HAP1 cell line is that it is near-haploid, meaning it contains only one copy for most of its chromosomes. The genome sequence of the parental KBM-7 cell line has been determined.[2] HAP1 cells have been used in several recent publications to study several aspects of cell biology.
Cultivation
HAP1 cells are adherent and maintained in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum. The doubling time of HAP1 cells is approximately 24 hours.


Loss of function analysis through endogenous gene knockouts is a powerful approach for understanding the cellular role of a protein. In standard diploid cell models, rapid generation of engineered cell lines is complicated by the need to knock out two alleles. As a result, the number of validated human cellular models available remains limited.
Haploid cellular models represent an attractive alternative for in vitro genetic analyses of molecular, cellular, and developmental events. Haploid cellular models are easier to generate than modified diploid cell lines as only one allele needs to be targeted to obtain a complete loss-of-function phenotype.

These cellular models have been generated predominantly in HAP1 cells, which is a near-haploid human adherent cell line that displays a fibroblast-like morphology. A detailed characterization of HAP1 cells including whole genome and RNA sequencing data has been published.

Spectral karyotyping and brightfield imaging of HAP1 cells
The relative simplicity of HAP1 cells allows Horizon to perform CRISPR-Cas9 based gene editing extremely efficiently, allowing us to offer rapidly expand our menu of off the shelf cell lines available.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 www.biovector.net [Supplier来源] http://www.biovector.net