mVenus-C1载体-BioVector NTCC Inc.

质粒名称:mVenus-C1

目录编号:54651

存储实验室:Michael Davidson

用途:C1克隆载体载体骨架基本信息载体名称:mVenus-C1载体抗性:卡那霉素空载体大小:4750 bp载体类型:哺乳细胞表达筛选标记:新霉素（G418）高拷贝/低拷贝:高拷贝生长条件:37 ℃插入基因信息插入基因名称:None标签/融合蛋白:mVenus（N端）文献引用方法材料和方法部分:mVenus-C1 was a gift from Michael Davidson (Addgene plasmid # 54651)载体质粒图谱和多克隆位点信息mVenus-C1

载体图谱

载体简介

载体序列LOCUS       mVenus-C1 Sequencing Result851 bp DNASYNDEFINITION  mVenus-C1 Sequencing ResultACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..851                     /organism="mVenus-C1 Sequencing Result"                     /mol_type="other DNA"     CDS             39..836                     /label="ORF frame 3"     gene            42..776                     /label="EYFP (variant)"                     /gene="EYFP (variant)"     misc_feature    complement(84..105)                     /label="EGFP_N_primer"     misc_feature    492..575                     /label="M153T,V163A,S175G (Venus)"     misc_feature    692..713                     /label="EGFP_C_primer"ORIGIN    1 GTGAACCGTC AGATCCGCTA GCGCTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC   61 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT  121 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGCTGA  181 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGGGCTACG  241 GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG  301 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA  361 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG  421 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA  481 GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA  541 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC  601 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCAAGC  661 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG  721 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGCTCAAG  781 CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA  841 TCATAATCAG C//