OCI-AML3 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

OCI-AML3 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 OCI-AML3 cell line Cat No.: NTCC596210 Cell line: OCI-AML3 NTCC no.: NTCC-ACC582 Species: human (Homo sapiens) Cell type: acute myeloid leukemia Origin: established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation Reference(s): 14870, 15050, 15051, 16666, 16667 Biosafety level: 1 Permissions and restrictions: A NTCC Cell Culture Data: Morphology: single, mostly round cells growing in suspension; image; Medium: 80% alpha-MEM + 20% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; at first cultivate in 12- or 24-well plates; split saturated culture 1:3 to 1:4 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 35-40 hours Harvest: maximum density at about 2.5 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO NTCC Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, cyCD68 +, HLA-DR -; image Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile Species: confirmed as human by cytogenetics and species PCR Cytogenetics: human hyperdiploid karyotype - 48(45-50)<2n>X/XY, +1, +5, +8, der(1)t(1;18)(p11;q11), i(5p), del(13)(q13q21), dup(17)(q21q25) - sideline with r(Y)x1-2 - hemizygous for RB1 Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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