pCas9cur (BioVector-071538) BioVector NTCC质粒载体菌种细胞基因保藏中心

pCas9cur (BioVector-071538) BioVector NTCC质粒载体菌种细胞基因保藏中心 Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
Vector backbone pSC101ts (Search Vector Database) Vector type Bacterial Expression, CRISPR GROWTH IN BACTERIA Bacterial Resistance(s) Chloramphenicol Growth Temperature 30°C Growth Strain(s) DH5alpha Copy number Unknown GENE/INSERT 1 Gene/Insert name Cas9 Species S. pyogenes GENE/INSERT 2 Gene/Insert name Plasmid curing system Alt name gRNA targeting bla gene Promoter pBAD CLONING INFORMATION FOR GENE/INSERT 2 Cloning method Unknown 5′ sequencing primer unknown
The plasmid curing system consists of an arabinose inducible promoter, PBAD to express a gRNA targeting the bla gene on the gRNA plasmid. When induced, the bla-targeting gRNA is expressed, leading Cas9 to cleave the gRNA plasmid and resulting in plasmid elimination.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话：+86-010-53513060 网址：www.biovector.net [Supplier来源] http://www.biovector.net