Saccharomyces cerevisiae (ATCC® 201447™) BioVector NTCC质粒载体菌种细胞基因保藏中心

Saccharomyces cerevisiae (ATCC® 201447™) BioVector NTCC质粒载体菌种细胞基因保藏中心 Strain Designation: AB1380 Deposited Name: Saccharomyces cerevisiae Hansen, teleomorph Genotype: MATa ade21 can1100 lys21 trp1289 ura352 his5 [psi+] Product Description: An ampoule containing viable cells suspended in cryoprotectant. The information recommended in this section is to assist users in obtaining living culture(s) for their studies. The recommendation does not imply that the conditions or procedures provided below are optimum. Experienced researchers may initiate the growth of a culture in their own way. ATCC® Medium 1245: YEPD Growth Conditions Temperature: 25.0°C Recommended Procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below 70° C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally 20° C). Storage of frozen material at this temperature will result in the death of the culture. 1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule. 2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 μL (or 23 agar cubes) of the content onto a plate or broth with medium recommended. 3. Incubate the inoculum/strain at the temperature and conditions recommended. 4. Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.
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