- BioVector NTCC典型培养物保藏中心
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pDEST15 vector BioVector NTCC质粒载体菌种细胞基因保藏中心 The E. coli Expression System with Gateway® Technology contains a series of Gateway®-adapted destination vectors designed to facilitate high-level, inducible expression of recombinant proteins in E. coli using the pET system. Depending on the vector chosen, the pDEST™ vectors allow production of native, N-terminal, or C-terminal-tagged recombinant proteins (see table below).
pDEST™15 and pDEST™17 are N-terminal fusion vectors and contain an ATG initiation codon upstream of the GST and 6XHis tags, respectively. In each vector, a Shine-Dalgarno RBS is included upstream of the initiation ATG to ensure optimal spacing for proper translation initiation in E. coli. Your gene of interest in the entry clone must:
• Be in frame with the N-terminal tag after recombination.
• Contain a stop codon.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话:+86-010-53513060 网址:www.biovector.net [Supplier来源] http://www.biovector.net
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话:+86-010-53513060 网址:www.biovector.net [Supplier来源] http://www.biovector.net
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