SG13009 E.coli大肠杆菌菌株感受态 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号:SG13009 E.coli大肠杆菌菌株感受态
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SG13009 E.coli大肠杆菌菌株感受态 BioVector NTCC质粒载体菌种细胞基因保藏中心 Description
SG13009 strain is derived from E. coli K12 and have the phenotype NaIS, StrS, RifS, Thi–, Lac–, Ara+, Gal+, Mtl–, F–, RecA+, Uvr+, Lon+.
The pREP4 plasmid is already present in the E. coli strain SG13009[pREP4].
Strain SG13009[pREP4] (Gottesman et al. 1981) is useful for the production of proteins that are poorly expressed in M15[pREP4].
Note that E. coli strain SG13009 does not harbor a chromosomal copy of the lacIq mutation, so pREP4 must be maintained by selection for kanamycin resistance.
Genotype analysis of E. coli strains SG13009 and M15
1. Both strains harbor a plasmid (pREP4) containing a gene that confers kanamycin resistance,Kmr.
2. Both strains are prototrophs and can grow on salt agar supplemented with 10 µg/ml thiamine-HCl. They do not require additional growth additives such as amino acids and nucleotide bases.
3. Both strains are sensitive to the following:
Streptomycin 100 µg/ml
Rifampicin 50 µg/ml
Nalidixic Acid 20 µg/ml
They therefore have no mutations in rpsL, rpoB, and gyrA.
4. Both strains cannot metabolize the following:
Lactose (Lac.)
Mannitol (Mtl.)
i. e. they contain mutations in the lac and mtl genes.
5. Data on other genes related to carbohydrate metabolism indicate that the TCA-cycle enzymes
correspond to those of the wild type.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话:+86-010-53513060 网址:www.biovector.net [Supplier来源] http://www.biovector.net
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话:+86-010-53513060 网址:www.biovector.net [Supplier来源] http://www.biovector.net
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