Staphylococcus aureus subsp. aureus (ATCC® 33591™) BioVector NTCC质粒载体菌种细胞基因保藏中心

Staphylococcus aureus subsp. aureus (ATCC® 33591™) BioVector NTCC质粒载体菌种细胞基因保藏中心 Designation: 328 Deposited Name: Staphylococcus aureus subsp. aureus Rosenbach Genotype: pvl negative Product Description: Used in testing antimicrobial handwashing formulations. This strain is methicillin resistant (MRSA). Medium ATCC® Medium 3: Nutrient agar or nutrient broth Growth Conditions Temperature: 37°C Atmosphere: Aerobic Propagation Procedure 1. Open vial according to enclosed instructions. 2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet. 3. Aseptically transfer this aliquot back into the broth tube. Mix well. 4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate. 5. Incubate the tubes and plate at 37°C for 24 hours. Absence of pvl gene confirmed by PCR. Purified genomic DNA of this strain is available as ATCC® 33591D5
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