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mCherry-Parkin BioVector NTCC质粒载体菌种细胞基因保藏中心 PURPOSE
Mammalian expression of human Park2 fused to mCherry
mCherry-C1 (Search Vector Database) Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA Bacterial Resistance(s) Kanamycin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name Park2 Alt name Parkin Species H. sapiens (human) Insert Size (bp) 1300 GenBank IDNM_004562 Entrez Gene PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ) Tag / Fusion Protein mCherry (N terminal on backbone) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site HindIII (not destroyed) 3′ cloning site BamH1 (not destroyed) 5′ sequencing primer n/a
first ATG is deleted
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话：+86-010-53513060 网址：www.biovector.net [Supplier来源] http://www.biovector.net
mCherry-C1 (Search Vector Database) Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA Bacterial Resistance(s) Kanamycin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name Park2 Alt name Parkin Species H. sapiens (human) Insert Size (bp) 1300 GenBank IDNM_004562 Entrez Gene PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ) Tag / Fusion Protein mCherry (N terminal on backbone) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site HindIII (not destroyed) 3′ cloning site BamH1 (not destroyed) 5′ sequencing primer n/a
first ATG is deleted
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 电话：+86-010-53513060 网址：www.biovector.net [Supplier来源] http://www.biovector.net
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