HLT013 E.coli大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HLT013 E.coli大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 工程菌HLT013: 出发菌株E.coli BL21
E.coli HLT013, a novel thymidine-producing strain of Escherichia coli was prepared by genome recombineering. Eleven genes were deleted by replacement with an expression cassette, and 7 genes were integrated into the genome. The resulting strain, E. coli HLT013, showed a high thymidine yield with a low deoxyuridine content. DNA microarrays were then used to compare the gene expression profiles of HLT013 and its isogenic parent strain. Based on microarray analysis, the pyrbiosynthesis genes and 10 additional genes were selected and then expressed in HLT013 to find reasonable candidates for enhancing thymidine yield. HLT013 was the base strain for finding reasonable thymidine-producing candidates. HLT013 has 11 disrupted genes (Δtdk, ΔpurR, Δung, ΔpepA, Δudp, ΔargR, ΔdeoABCD, and rpoS) and 7 overexpressed genes (T4 intron-deleted version of the td gene [tdΔI], the phage PBS2 TMPase gene, T4nrdCA, and mature T4 nrdB gene [nrdBmat], udk, and dcd)
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