FADU cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

FADU cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line: FADU DSMZ no.: ACC 784Species: human (Homo sapiens)Cell type: pharynx carcinoma Origin: established in 1968 from a squamous cell carcinoma of the hypopharynx grade II of a 56-year-old Indian man; cells were described in the literature to overexpress bcl-1 and to be tumorigenic in mice Reference(s): 24158, 14537, 14529Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:epithelioid cells growing adherently as monolayerMedium: 90% Dulbecco's MEM + 10% h.i. FBS Subculture: seed out at ca. 1.5 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once a week using trypsin/EDTA Incubation: at 37 °C with 5% CO2 Doubling time: ca. 30 hours Harvest: cell harvest of ca. 30-35 x 106 cells/175 cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in PCR assay Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP (+), neurofilament -, vimentin - Fingerprint: genetic typing showed a short tandem repeat (STR) profile indicated as unique by a search of the international STR database Species: confirmed as human by STR-typing Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HPV -, HTLV-I/-II -, MLV -
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