Alloprevotella rava BioVector NTCC质粒载体菌种细胞基因保藏中心

Alloprevotella rava BioVector NTCC质粒载体菌种细胞基因保藏中心 Name:
Alloprevotella rava Downes et al. 2013
NTCC No.:
22548T
Strain designation:
81/4 12
Isolated from:
human periodontitis patient, oral cavity, subgingival plaque (>8 mm periodontal pocket)
Geographic origin:
United Kingdom, Cardiff
History:
<- J. Downes, King's College London, United Kingdom; 81/4 12 <- W. G. Wade
Genbank accession numbers:
16S rRNA gene: JQ039189
Cultivation conditions:
Medium COLUMBIA BLOOD MEDIUM , anaerobic, 37°C

COLUMBIA BLOOD MEDIUM
Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets
of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below).
545. TRYPTONE SOYA BROTH (TSB)
Peptone from casein 17.0 g
Peptone from soymeal 3.0 g
D(+)-Glucose 2.5 g
NaCl 5.0 g
K2HPO4 2.5 g
Distilled water 1000.0 ml
Adjust pH to 7.3.
The same as Caso Bouillon (Merck or Oxoid, Germany).
215. BHI MEDIUM
Brain Heart Infusion (Difco) 37.0 g
Distilled water 1000.0 ml
1. NUTRIENT BROTH
Peptone 5.0 g
Meat extract 3.0 g
Distilled water 1000.0 ml
Adjust pH to 7.0.

Incubation time: 2-3 days
Summary and
additional information:
<- J. Downes, King's College London, United Kingdom; 81/4 12 <- W. G. Wade. Human periodontitis patient, oral cavity, subgingival plaque (>8 mm periodontal pocket); United Kingdom, Cardiff. Type strain.Taxonomy/description (17630). Sequence accession no. 16S rRNA gene: JQ039189.
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
Downes, J., Dewhirst, F. E., Tanner, A. C., Wade, W. G. (2013). Description ofAlloprevotella rava gen. nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov. Int J Syst Evol Microbiol 63 ( Pt 4 ): 1214-8 .http://www.ncbi.nlm.nih.gov/pubmed/22753527

Risk group:
1


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