IgK-IFN-luc BioVector NTCC质粒载体菌种细胞基因保藏中心

IgK-IFN-luc BioVector NTCC质粒载体菌种细胞基因保藏中心 Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
Contains two copies of the immunoglobulin kappa light chain kappa-B site (5'-GGGGACTTTCC-3') upstream of the interferon-beta minimal promoter (–55 to +19) (Fujita et al., 1987) driving luciferase expression.
Backbone size w/o insert (bp) 5800
Vector type Mammalian Expression, Luciferase
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name IgK-IFN
Alt name IFNB1
Alt name IgK2‐IFN‐LUC reporter
Species H. sapiens (human)
Insert Size (bp) 80
Entrez Gene IFNB1 (a.k.a. IFB, IFF, IFN-beta, IFNB)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site SacII (not destroyed)
3′ cloning site HindIII (not destroyed)
5′ sequencing primer na
3′ sequencing primer LucNrev
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
BioVector-014886 [Supplier来源] http://www.biovector.net