pMonAID_dCas9-PmCDA_Hyg_ALS BioVector NTCC质粒载体菌种细胞基因保藏中心

pMonAID_dCas9-PmCDA_Hyg_ALS

Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

Vector backbone pZDgRNA_Cas9ver.2 (D10A)_HPT

(Search Vector Database)

Backbone size w/o insert (bp) 17773

Vector type Plant Expression

Selectable markers Hygromycin

GROWTH IN BACTERIA

Bacterial Resistance(s) Spectinomycin

Growth Temperature 37°C

Growth Strain(s) DH5alpha

Copy number Unknown

GENE/INSERT

Gene/Insert name SpCas9

Species Streptococcus pyogenes

Mutation D10A and H840A for dead Cas9

Promoter 2x35S

Tag / Fusion Protein

PmCDA1 (C terminal on insert)

CLONING INFORMATION

Cloning method Gibson Cloning

5′ sequencingprimer ggaagttcatttcatttggagag

3′ sequencingprimer ccatttgcattttgatgtccg

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