Penicillium chrysogenum ATCC 12689 BioVector NTCC质粒载体菌种细胞基因保藏中心

Penicillium chrysogenum
ATCC 12689 BioVector NTCC质粒载体菌种细胞基因保藏中心 Strain Designation: Wis. 5120
Deposited Name: Penicillium chrysogenum Thom, anamorph
Product Description:
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
The information recommended in this section is to assist users in obtaining living culture(s) for their studies.
The recommendation does not imply that the conditions or procedures provided below are optimum.
Experienced researchers may initiate the growth of a culture in their own way.
ATCC® Medium 325: Malt extract agar (Blakeslee's formula)
Growth Conditions
Temperature: 26.0°C
Recommended Procedure
For freezedry
(lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a
sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight)
rehydration might increase viability of some fungi..
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid
or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 12
days
of incubation. However, the time necessary for significant growth will vary from strain to strain.
produces penicillin; produces high yield of nonpigmented penicillin.
No DNA sequencing was performed in house on this product.
Selected from Wis. 50935
which was derived by sequential Nmustard
exposure of Wis. 5025,
Wis. 492166
and Wis. 49133,
see also ATCC 11709.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
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