SMD1168H strain毕赤酵母菌株表达宿主菌 BioVector NTCC质粒载体菌种细胞基因保藏中心

SMD1168H strain毕赤酵母菌株表达宿主菌 BioVector NTCC质粒载体菌种细胞基因保藏中心 SMD1168H strain

Description
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest.
Table 2 provides information to help you choose the appropriate strain
SMD1168H (pep4)
Application
Selection of Zeocin resistant expression vectors to generate strains without protease A activity.

Recovery
1. Obtain an YPD agar plate.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 30 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

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