pREDTKI BioVector NTCC质粒载体菌种细胞基因保藏中心

pREDTKI

BioVector NTCC质粒载体菌种细胞基因保藏中心

大肠杆菌高效基因修改编辑载体，原核表达Red重组酶和I-SceI内切酶，利用Lambda Red重组法和I-SceI内切酶切割实现大肠杆菌高效基因修改与编辑。pKD46载体骨架。

Map图谱：

E coli Genetic Modification
pKD46
(Search Vector Database)
Backbone size w/o insert (bp) 6300
Modifications to backbone replace amp to kan
Vector type Bacterial Expression ; Red recombinase and I-SceI endonuclease
GROWTH IN BACTERIA

Bacterial Resistance(s) low Kan
Growth Temperature 30°C
Growth Strain(s) DH5alpha
Growth instructions Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
Copy number Low Copy
GENE/INSERT

Gene/Insert name I-SceI
Alt name mitochondrial intron of Saccharomyces cerevisiae
Species S. cerevisiae (budding yeast)
Insert Size (bp) 3000
Entrez Gene SCEI (a.k.a. Q0160)
Promoter trc
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site NcoI (not destroyed)
3′ cloning site NcoI (not destroyed)
5′ sequencing primer none
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net      [Supplier来源]  http://www.biovector.net