pksdiacas9-sgrna BioVector NTCC质粒载体菌种细胞基因保藏中心

pksdiacas9-sgrna

BioVector NTCC质粒载体菌种细胞基因保藏中心  

Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter
Vector backbone pBlueScript KS(+)
(Search Vector Database)
Backbone manufacturer Stratagene
Backbone size w/o insert (bp) 2904
Total vector size (bp) 8577
Modifications to backbone Bsa1 site located in ampicillin resistance gene removed
Vector type CRISPR
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT 1

Gene/Insert name LHCF2 promoter
Species Phaeodactylum tricornutum
Insert Size (bp) 442
GenBank IDZ24761.1
Promoter Cas9 module
CLONING INFORMATION FOR GENE/INSERT 1

Cloning method Restriction Enzyme
5′ cloning site Sac1 (not destroyed)
3′ cloning site Xba1 (not destroyed)
5′ sequencing primer none
3′ sequencing primer none
(Common Sequencing Primers)
GENE/INSERT 2

Gene/Insert name diaCas9
Species Synthetic
Insert Size (bp) 4283
Promoter Cas9 module
CLONING INFORMATION FOR GENE/INSERT 2

Cloning method Restriction Enzyme
5′ cloning site Xba1 (not destroyed)
3′ cloning site BamHI (not destroyed)
5′ sequencing primer none
3′ sequencing primer none
(Common Sequencing Primers)
GENE/INSERT 3

Gene/Insert name LHCF1 terminator
Species Phaeodactylum tricornutum
Insert Size (bp) 216
GenBank IDZ24761.1
Promoter Cas9 module
CLONING INFORMATION FOR GENE/INSERT 3

Cloning method Restriction Enzyme
5′ cloning site BamHI (not destroyed)
3′ cloning site Pst1 (not destroyed)
5′ sequencing primer none
3′ sequencing primer none
(Common Sequencing Primers)
GENE/INSERT 4

Gene/Insert name U6 promoter
Species Phaeodactylum tricornutum
Insert Size (bp) 280
GenBank IDCM000611.1
Promoter sgRNA module
CLONING INFORMATION FOR GENE/INSERT 4

Cloning method Restriction Enzyme
5′ cloning site Pst1 (not destroyed)
3′ cloning site none (unknown if destroyed)
5′ sequencing primer none
3′ sequencing primer none
(Common Sequencing Primers)
GENE/INSERT 5

Gene/Insert name sgRNA
Species Synthetic
Insert Size (bp) 103
Promoter sgRNA module
CLONING INFORMATION FOR GENE/INSERT 5

Cloning method Unknown
5′ sequencing primer none
3′ sequencing primer none
(Common Sequencing Primers)
GENE/INSERT 6

Gene/Insert name U6 3' region
Species Phaeodactylum tricornutum
Insert Size (bp) 330
GenBank IDCM000611.1
Promoter sgRNA module
CLONING INFORMATION FOR GENE/INSERT 6

Cloning method Restriction Enzyme
5′ cloning site none (unknown if destroyed)
3′ cloning site HindIII (not destroyed)
The diaCas9 module was synthesized by GeneArt® Services Thermo Fisher Scientific Inc.
The sgRNA module was synthesized by Eurofins Genomic Services Ltd.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net      [Supplier来源]  http://www.biovector.net