牙龈卟啉单胞菌Porphyromonas gingivalis BioVector NTCC质粒载体菌种细胞基因保藏中心

牙龈卟啉单胞菌Porphyromonas gingivalis BioVector NTCC质粒载体菌种细胞基因保藏中心 牙龈卟啉单胞菌Porphyromonas gingivalis (Coykendall et al. 1980) Shah and Collins 1988
Synonym(s):
Bacteroides gingivalis Coykendall et al. 1980
NTCC No.:
20709, Type strain
Strain designation:
2561
Isolated from:
human gingival sulcus
Geographic origin:
country of origin unknown
History:
<- ATCC <- A.I. Coykendall <- J. Slots, 2561
Genbank accession numbers:
16S rRNA gene: AB035457
Cultivation conditions:
Medium CHOPPED MEAT MEDIUM WITH CARBOHYDRATES + haemin + menadione (Vit K3), anaerobic, 37°C

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
Ground beef (fat free) 500.0 g
Distilled water 1000.0 ml
NaOH 1 N 25.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix
meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature,
skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate
add water to a final volume of 1000 ml, and then add:
Casitone 30.0 g
Yeast extract 5.0 g
K2HPO4 5.0 g
Na-resazurin solution (0.1% w/v) 0.5 ml
D-Glucose 4.0 g
Cellobiose 1.0 g
Maltose 1.0 g
Starch (soluble) 1.0 g
To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l Lcysteine
hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml
medium into Hungate-type tubes (for strains demanding meat particles put those first
into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121C for 30
min. For agar slants use 15.0 g agar per 1000.0 ml medium.
In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or
Vitamin K3 is necessary. Add to 1000.0 ml of medium after autoclaving:
Haemin solution (see below) 10.00 ml
Vitamin K1 or Vitamin K3 solution (see below) 10.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and
filter sterilize. Store refrigerated.
Vitamin K1 solution:
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.
Vitamin K3 solution:
Dissolve 5 mg/ml of vitamin K3 in 95% ethanol, dilute to 0.05 mg/ml in water and filter
sterilize. Store refrigerated in a brown bottle.
Summary and
additional information:
<- ATCC <- A.I. Coykendall <- J. Slots, 2561. Human gingival sulcus. Type strain. Taxonomy/description (3251, 3326). Sequence accession no. 16S rRNA gene: AB035457. (Medium 110 + haemin + menadione (Vit K3), 37°C, anaerobic or Medium 693, 37°C, anaerobic). It may take more than 10 days to show black pigmentation.
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
3251, 3326, 3327
Risk group:
2


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