粘性放线菌Actinomyces oris BioVector NTCC质粒载体菌种细胞基因保藏中心

粘性放线菌Actinomyces oris BioVector NTCC质粒载体菌种细胞基因保藏中心 粘性放线菌Actinomyces oris Henssge et al. 2009
NTCC No.:
23056, Type strain
Strain designation:
W1053, WVU 474
Isolated from:
human sputum
Geographic origin:
country of origin unknown
History:
<- CCUG <- P. Pienta, ATCC <- L. K. Georg, CDC; W1053 <- New York City Dept. Hlth.; WVU 474
Genbank accession numbers:
16S rRNA gene: EU667403
Cultivation conditions:
PYG MEDIUM (modified) add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch, anaerobic, 37°C

PYG MEDIUM (modified)
Trypticase peptone 5.00 g
Peptone 5.00 g
Yeast extract 10.00 g
Beef extract 5.00 g
Glucose 5.00 g
K2HPO4 2.00 g
Tween 80 1.00 ml
Cysteine-HCl x H2O 0.50 g
Resazurin 1.00 mg
Salt solution (see below) 40.00 ml
Distilled water 950.00 ml
Haemin solution (see below) 10.00 ml
Vitamin K1 solution (see below) 0.20 ml
The vitamin K1, haemin solution and the cysteine are added after the medium has been
boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and
autoclave.
Salt solution:
CaCl2 x 2 H2O 0.25 g
MgSO4 x 7 H2O 0.50 g
K2HPO4 1.00 g
KH2PO4 1.00 g
NaHCO3 10.00 g
NaCl 2.00 g
Distilled water 1000.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store
refrigerated.
Vitamin K1 solution:
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.
Summary and
additional information:
<- CCUG <- P. Pienta, ATCC <- L. K. Georg, CDC; W1053 <- New York City Dept. Hlth.; WVU 474. Human sputum. Type strain. Taxonomy/description (13129). Sequence accession no. 16S rRNA gene: EU667403. (Medium 104 add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch, 37°C, anaerobic).
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
13129
Risk group:
2


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