Prevotella intermedia中间普氏菌 BioVector NTCC质粒载体菌种细胞基因保藏中心

Prevotella intermedia中间普氏菌 BioVector NTCC质粒载体菌种细胞基因保藏中心 Prevotella intermedia (Holdeman and Moore 1970) Shah and Collins 1990
中间普氏菌
Cat #: NTCC597219
Synonym(s):
Bacteroides intermedius (Holdeman and Moore 1970) Johnson and Holdeman 1983
Bacteroides melaninogenicus subsp. intermedius Holdeman and Moore 1970
NTCC No.:
20706, Type strain
Strain designation:
B422, VPI 4197
Isolated from:
empyema
Geographic origin:
country of origin unknown
History:
L.V. Holdeman, VPI 4197 <- S. Finegold, B422
Genbank accession numbers:
16S rRNA gene: FJ792535
Cultivation conditions:
Medium PYG MEDIUM (modified) , anaerobic, 37°C

PYG MEDIUM (modified)
Trypticase peptone 5.00 g
Peptone 5.00 g
Yeast extract 10.00 g
Beef extract 5.00 g
Glucose 5.00 g
K2HPO4 2.00 g
Tween 80 1.00 ml
Cysteine-HCl x H2O 0.50 g
Resazurin 1.00 mg
Salt solution (see below) 40.00 ml
Distilled water 950.00 ml
Haemin solution (see below) 10.00 ml
Vitamin K1 solution (see below) 0.20 ml
The vitamin K1, haemin solution and the cysteine are added after the medium has been
boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and
autoclave.
Salt solution:
CaCl2 x 2 H2O 0.25 g
MgSO4 x 7 H2O 0.50 g
K2HPO4 1.00 g
KH2PO4 1.00 g
NaHCO3 10.00 g
NaCl 2.00 g
Distilled water 1000.00 ml
Haemin solution:
Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store
refrigerated.
Vitamin K1 solution:
Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated
in a brown bottle.
Summary and
additional information:
L.V. Holdeman, VPI 4197 <- S. Finegold, B422. Empyema. Type strain. Taxonomy/description (1300, 3249, 3923). Sequence accession no. 16S rRNA gene: FJ792535. Quality control strain according to DIN 58959-7 (6924). Dark pigment. (Medium 104, 37°C, anaerobic or Medium 693, 37°C, anaerobic or Medium 110, 37°C, anaerobic).
For detailed information:
BacDive - The Bacterial Diversity Metadatabase
Literature:
1300, 3249, 3923, 6924
Risk group:
2


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