pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统 BioVector NTCC质粒载体菌种细胞基因保藏中心

pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统

BioVector NTCC质粒载体菌种细胞基因保藏中心                      

pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统，pUC18复制起始位点，温度敏感型pE194ts复制子，Kan抗性基因，Pman启动子+Cas9基因，PvavP启动子+sgRNA向导序列，lacPOZ'，λ oop终止子，以及T7启动子。

CRISPR-Cas9 vector pJOE8999. (A) Physical map of vector pJOE8999 containing the pUC18 minimal origin, the temperature-sensitive replication origin from pE194ts, a kanamycin resistance gene (kanR), cas9 under the control of the PmanP promoter, the sgRNA transcribed from the semisynthetic promoter PvanP* interrupted by the lacZ α fragment (lacPOZ'), the λ oop terminator, and the T7 promoter (T7P). (B) Cloning sites in the vector pJOE8999. (C) Promoter sequence (PvanP*) upstream of the sgRNA with the −35 and −10 sequences and the BsaI restriction sites (bold) for insertion of the spacer sequence.
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