RCASBP (E) (CC#36)
(BioVector-015163) BioVector NTCC质粒载体菌种细胞基因保藏中心

RCASBP (E) (CC#36)
(BioVector-015163) BioVector NTCC质粒载体菌种细胞基因保藏中心 Vector backbone RCASBP (E)
(Search Vector Database)
Backbone size (bp) 12000
Vector type Retroviral ; Avian expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Unknown
GENE/INSERT

Gene/Insert name None
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ sequencing primer n/a
(Common Sequencing Primers)
RESOURCE INFORMATION

A portion of this plasmid was derived from a plasmid made by RCASBP(A) provided by S. Hughes.
Terms and Licenses
UBMTA
DEPOSITOR COMMENTS

The name RCAS stands for Replication-Competent ASLV long terminal repeat (LTR) with a Splice acceptor. The RCAS vectors retain the src splice site and express an inserted gene via a spliced message. These vectors will replicate in appropriately chosen avian cells (usually chicken or quail, although others can be used). In some cases, RCAS vectors will infect, but will not replicate, in mammalian cells. Please also see the paper Hughes SH et al., J Virol 1987 Oct; 61(10):3004-3012.

We constructed a fourth vector of the RCAS series of the E subgroup. Two hypervariable regions within the extracellular domain of the env gene are of particular importance in determining the host range of different viral subgroups. Starting with RCASBP(A), a new vector of the E subgroup, called RCASBP(E), was constructed by swapping a portion of the A env with E env. The E env sequences were obtained from pRAV-0 by KpnI-SalI digestion and isolation of the 1.1-kb env fragment. This fragment was ligated to RCASBP(A) in place of the homologous KpnI-SalI fragment.
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