BA/F3 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心

BA/F3 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心 BA/F3 Cells

Cell line:
BA/F3
DSMZ no.:
ACC 300
Species:
murine (mouse) (Mus musculus)
Cell type:
pro B cells
Origin:
IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.
Reference(s):
14829, 14830
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
mostly single, round (some polymorph) cells in suspension (or occasionally in clumps); image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml mouse IL-3 or 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26)
Subculture:
split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105cells/ml; at high cell density (>2 x 106 cells/ml), a cytokine-independent subclone may grow out relatively quickly. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20 hours
Harvest:
maximal density of about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD11b (+), F4/80 (+); image
Species:
originally confirmed as mouse with IEF of AST, MDH, NP, PEP B and with species PCR and recently as mus musculus by COI DNA Barcoding
Cytogenetics:
murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -

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