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pFC333 BioVector NTCC质粒载体菌种细胞基因保藏中心 AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
Total vector size (bp) 15667
Vector type Bacterial Expression, CRISPR ; Fungal expression
Selectable markers Zeocin ; bleomycin, phleomycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Unknown
GENE/INSERT 1
Gene/Insert name Cas9
Species Synthetic; synthetic gene, codon optimized for A. niger
Insert Size (bp) 4131
GenBank IDKT031983
Promoter Aspergillus nidulans tef1 promoter
Tag / Fusion Protein
SV40 NLS (C terminal on insert)
CLONING INFORMATION FOR GENE/INSERT 1
Cloning method Unknown
5′ sequencing primer CTTCTCTGCTCAGCACCTCTACG
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert name ble (bleomycin resistance marker)
Insert Size (bp) 375
Promoter Aspergillus nidulans trpC promoter
CLONING INFORMATION FOR GENE/INSERT 2
Cloning method Unknown
5′ sequencing primer GCTAGTGGAGGTCAACACATCAATGC
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net [Supplier来源] http://www.biovector.net
Total vector size (bp) 15667
Vector type Bacterial Expression, CRISPR ; Fungal expression
Selectable markers Zeocin ; bleomycin, phleomycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Unknown
GENE/INSERT 1
Gene/Insert name Cas9
Species Synthetic; synthetic gene, codon optimized for A. niger
Insert Size (bp) 4131
GenBank IDKT031983
Promoter Aspergillus nidulans tef1 promoter
Tag / Fusion Protein
SV40 NLS (C terminal on insert)
CLONING INFORMATION FOR GENE/INSERT 1
Cloning method Unknown
5′ sequencing primer CTTCTCTGCTCAGCACCTCTACG
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert name ble (bleomycin resistance marker)
Insert Size (bp) 375
Promoter Aspergillus nidulans trpC promoter
CLONING INFORMATION FOR GENE/INSERT 2
Cloning method Unknown
5′ sequencing primer GCTAGTGGAGGTCAACACATCAATGC
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net [Supplier来源] http://www.biovector.net
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