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po1g 解脂耶氏酵母 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:po1g 解脂耶氏酵母
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Yarrowia lipolytica Po1g strain 解脂耶氏酵母

BioVector NTCC质粒载体菌种细胞基因保藏中心                      


Yarrowia lipolytica Po1g strain is a derivative of the wild-type strain W29 (ATCC 20460) by a series of genetic modifications.
The cells were maintained on a sterilized yeast-, peptone-, and dextrose- (YPD-) agar plate containing 10 g/L yeast extract (Bacto, France), 10 g/L peptone (Bacto, France), 20 g/L glucose (Acros Organics, USA), and 20 g/L agar (Acros Organics, USA) at 4°C for further microbial fermentation.

Application transformation host
Medium YPD Medium
Broth Medium
YPD Broth(BD 242820)……………………....50 g
DI Water………………………………………..1000 ml
Or for solid medium:
Agar Medium
YPD Agar(BD 242720)………………………..65 g
DI Water………………………………………...1000 ml
Dispense into appropriate vessel and autoclave at 121ºC.
*YPD is commercially available from BD. To make medium from scratch, follow
formulation below:
Yeast Extract……………………………………10.0 g
Peptone…………………………………………20.0 g
Dextrose………………………………………...20.0 g
Agar (if needed)………………………………..20.0 g
DI Water………………………………………...1000 ml
Adjust for final pH of 5.6. Autoclave at 121oC.
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic

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