NB-4 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NB-4 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line:
NB-4
Species:
human (Homo sapiens)
Cell type:
acute promyelocytic leukemia
Origin:
established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
single cells in suspension, round and polymorphic cells, very few are giant cells; image; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5-1 x 106 cells/ml; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-45 hours
Harvest:
cell harvest of 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD11b -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD38 +, HLA-DR -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human hypertriploid karyotype with 3% polyploidy - 78(71-81)<3n>XX, -X, +2, +6, +7, +7, +11, +12, +13, +14, +17, -19, +20, +4mar, der(8)t(8;?)(q24;?), der(11)t(11;?)(?->::11p15->11q22.1::11q13->22.1:), der(12)t(12;?)(p11;?), 14p+, t(15;17)(q22;q11-12.1), der(19)t(10;19)(q21.1;p13.3)x2 - identity confirmed
Molec. Genetics:
expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -

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