pSIN4-CMV-K2M诱导多能干细胞慢病毒表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSIN4-CMV-K2M诱导多能干细胞慢病毒表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pSIN4-CMV-K2M诱导多能干细胞慢病毒质粒，CMV启动子同时表达Klf4和cMyc，与pSIN4-EF2-O2S and pSIN4-EF2-N2L配套使用比原有的四质粒体系具有更高的重编程效率。
After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. Infections with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-EF2-N2L have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
Vector backbone pSIN4-EF2-IRES-Pur
(Search Vector Database)
Backbone size w/o insert (bp) 5500
Vector type Mammalian Expression, Lentiviral ; reprogramming
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number Low Copy
GENE/INSERT

Gene/Insert name Klf4, cMyc
Species H. sapiens (human)
Insert Size (bp) 2733
GenBank IDNM_004235.2 NM_002467
Entrez Gene MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site NheI (not destroyed)
3′ cloning site SpeI (not destroyed)
5′ sequencing primer EF1a_for
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
[Supplier来源]BioVector NTCC Inc.
【Website网址】 http://www.biovector.net