Gryphon Retroviral Expression System I BioVector NTCC质粒载体菌种细胞基因保藏中心

Gryphon Retroviral Expression System I BioVector NTCC质粒载体菌种细胞基因保藏中心
The Gryphon retroviral system is an upgraded version of
the Phoenix™ Retroviral System, previously provided
by Orbigen. This system has been used in over 2500
laboratories worldwide for delivery of genes and libraries
to cells for biomedical research. An Ecotropic packaging
system or an Amphotropic system is currently available
for the delivery of genes to most dividing mammalian cell
types. The Retrovirus Expression Kit includes: an Ecotropic,
an Amphotropic packaging cell line, and two retroviral
expression vectors for high-level protein expression in most
mammalian cells.
Gryphon retrovirus producer lines, Gryphon Ecotropic
and Gryphon Amphotropic, for the generation of helper
free ecotropic and amphotropic retroviruses are based on
the 293T cell line, which is highly transfectable using either
calcium phosphate mediated transfection (see section VI)
or lipid-transfection protocols. These retrovirus producer
lines were created by placing constructs into 293T cells
capable of producing gag-pol and envelope protein for
ecotropic and amphotropic viruses. They are also selected
for stable growth and accessibility to transfection independently
from the previous Phoenix Eco and Ampho packaging
cells. The lines offer many advantages over previous
stable systems and these have led to their broad application
worldwide.
Features：
1. Virus can be produced in just a few days.
2. By introducing an IRES-CD8 surface marker downstream
of the reading frame of the gag-pol construct, CD8 expression
is a direct reflection of intracellular gag-pol and the stability
of the producer cell population’s ability to produce gag-pol
can be readily monitored by flow cytometry.
3. To prevent potential recombination, different non-Moloney
promoters for gag-pol and envelope were used.
4. Both lines are capable of carrying episomes for long-term
stable retrovirus production.
5. Helper virus production in these lines have been extensively
tested and found to be helper-virus free.
6. Alleleustrious mWasabi is a monomeric green fluorescent
protein that can be easily detected using standard GFP filter
sets. mWasabi may be used as a direct replacement for
EGFP or other GFPs for superior performance, and may be
co-imaged with blue and red fluorescent labels without substantial
bleed-through.

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