pC014 - LwCas13a-msfGFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pC014 - LwCas13a-msfGFP BioVector NTCC质粒载体菌种细胞基因保藏中心
Expresses active LwCas13a for mammalian RNA targeting
one lenti(AMP)
(Search Vector Database)
Backbone size w/o insert (bp) 9204
Total vector size (bp) 13584
Vector type Mammalian Expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) NEB Stable
Copy number High Copy
GENE/INSERT

Gene/Insert name LwCas13a-msfGFP
Alt name LwCas13a
Alt name LwC2c2
Alt name Cas13a
Species H. sapiens (human); Leptotrichia wadei
Insert Size (bp) 4380
Promoter EF1a
Tags / Fusion Proteins
msfGFP (C terminal on insert)
HA (C terminal on insert)
NLS (N terminal on insert)
NLS (C terminal on insert)
CLONING INFORMATION

Cloning method Gibson Cloning
5′ sequencing primer EF-1a Forward
3′ sequencing primer WPRE-R
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
[Supplier来源]BioVector NTCC Inc.
【Website网址】 http://www.biovector.net