AD-293 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心

AD-293 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心
s AD-293 cell line is a derivative of the commonly used HEK293 cell line, with improved cell adherence and plaque
formation properties. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5 DNA.1
AD-293
cells, like HEK293 cells, produce the adenovirus E1 gene in trans, allowing the production of infectious virus particles when cells
are transfected with E1-deleted adenovirus vectors such as the pAdEasy™-1 vector. Standard HEK293 cells do not adhere well to
tissue culture dishes, hindering adherent cell culture and plaque assay procedures. AD-293 cells demonstrate improved adherence
to tissue culture dishes, making AD-293 cell monolayers less susceptible to disruption during cell passaging and plaque assays.
Growth Medium
DMEM (containing 4.5 g/L glucose and 110 mg/L sodium
pyruvate and 2 mM L-glutamine), supplemented with 10% (v/v)
heat-inactivated fetal bovine serum
Freezing Medium (100 ml)
50 ml DMEM (containing 4.5 g/L glucose, 110 mg/L sodium
pyruvate and 2 mM L-glutamine)
40 ml heat-inactivated fetal bovine serum
10 ml dimethylsulfoxide (DMSO)
Filter sterilize
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net