BPK764 BioVector NTCC质粒载体菌种细胞基因保藏中心

BPK764 BioVector NTCC质粒载体菌种细胞基因保藏中心
Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
Use BsaI sites to insert sgRNA spacer sequence into the vector.
Vector backbone pACYCDuet-1
(Search Vector Database)
Backbone manufacturer EMD Millipore
Vector type Bacterial Expression, CRISPR
GROWTH IN BACTERIA

Bacterial Resistance(s) Chloramphenicol
Growth Temperature 37°C
Growth Strain(s) XL1 Blue
Copy number Low Copy
GENE/INSERT

Gene/Insert name mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
Promoter T7 (x2)
Tags / Fusion Proteins
NLS (C terminal on insert)
3x FLAG (C terminal on insert)
CLONING INFORMATION

Cloning method Gibson Cloning
5′ sequencing primer ACYC-DuetUP1
3′ sequencing primer oBK390 (5'-GCAAATTCGACCCGGTCGTCG-3')
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net