pIC20H-RL BioVector NTCC质粒载体菌种细胞基因保藏中心

pIC20H-RL BioVector NTCC质粒载体菌种细胞基因保藏中心
LOCUS JX668229 9499 bp DNA circular SYN 31-MAY-2013
DEFINITION TREX vector pIC20H-RL, complete sequence.
ACCESSION JX668229
VERSION JX668229.1
KEYWORDS .
SOURCE TREX vector pIC20H-RL
ORGANISM TREX vector pIC20H-RL
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9499)
AUTHORS Loeschcke,A., Markert,A., Wilhelm,S., Wirtz,A., Rosenau,F.,
Jaeger,K.E. and Drepper,T.
TITLE TREX: A Universal Tool for the Transfer and Expression of
Biosynthetic Pathways in Bacteria
JOURNAL ACS Synth Biol 2 (1), 22-33 (2013)
PUBMED 23656323
REFERENCE 2 (bases 1 to 9499)
AUTHORS Loeschcke,A., Markert,A., Drepper,T. and Jaeger,K.-E.
TITLE Direct Submission
JOURNAL Submitted (11-SEP-2012) Institute of Molecular Enzyme Technology,
Heinrich-Heine-University, Stetternicher Forst, Juelich, NRW 52426,
Germany
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9499
/organism="TREX vector pIC20H-RL"
/mol_type="other DNA"
/db_xref="taxon:1256640"
regulatory complement(274..295)
/regulatory_class="promoter"
/note="PT7; T7 RNA polymerase-specific promoter"
gene 326..1120
/gene="aphII"
CDS 326..1120
/gene="aphII"
/note="kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside 3'-phosphotransferase"
/protein_id="AFW89950.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 1178..1196
/note="OE; outside end of transposon Tn5"
oriT 1247..2438
/note="origin of conjuational transfer"
gene 2537..3803
/gene="tetR"
CDS 2613..3803
/gene="tetR"
/codon_start=1
/transl_table=11
/product="tetracycline export protein"
/protein_id="AFW89947.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
misc_feature complement(3861..3879)
/note="OE; outside end of transposon Tn5"
gene 3890..5383
/gene="tnp"
CDS 3953..5383
/gene="tnp"
/codon_start=1
/transl_table=11
/product="transposase"
/protein_id="AFW89948.1"
/translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
SITISSEGSEAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDV
ESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
ITLNAVLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
EGWEALQSKLDGFLAAKDLMAQGIKI"
gene complement(5483..6233)
/gene="aacC1"
CDS complement(5483..6016)
/gene="aacC1"
/note="getamicin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside acetyltransferase"
/protein_id="AFW89949.1"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
GIREEVMHFDIDPSTAT"
gene complement(6296..7015)
/gene="yfp"
CDS complement(6296..7015)
/gene="yfp"
/note="fluorescence reporter"
/codon_start=1
/transl_table=11
/product="yellow fluorescent protein"
/protein_id="AFW89951.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory 7024..7045
/regulatory_class="promoter"
/note="PT7; T7 RNA polymerase-specific promoter"
misc_feature 7050..7069
/note="lacO, lac operator"
gene 7698..8558
/gene="bla"
CDS 7698..8558
/gene="bla"
/note="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AFW89952.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"

【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net