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Lewis cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:Lewis cell line细胞株
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Lewis cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Lewis cell line
Cat No.: NTCC690441

Organism Mus musculus, mouse
Tissue lung
Product Format frozen
Morphology rounded - loosely attached or floating
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Lewis lung carcinoma
Strain C57BL
Applications
This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents
Derivation
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Antigen Expression
H-2b
Tumorigenic Yes
Effects
Yes, in C57BL mice
Comments
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.
The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent.
Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing
Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Population Doubling Time 21 hrs

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