pMD2.G慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.

LOCUS       pMD2.G	5824 bp 	DNA	SYN
DEFINITION  pMD2.G
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="pMD2.G"
                     /mol_type="other DNA"
     misc_feature    complement(40..59)
                     /label="SV40pro_F_primer"
     promoter        162..738
                     /label="CMV_immearly_promoter"
     misc_feature    241..528
                     /label="CAG_enhancer"
     misc_feature    695..715
                     /label="CMV_fwd_primer"
     promoter        696..765
                     /label="CMV_promoter"
     promoter        708..827
                     /label="CMV2_promoter"
     misc_feature    739..758
                     /label="pCEP_fwd_primer"
     misc_feature    741..765
                     /label="LNCX_primer"
     misc_feature    1015..1398
                     /label="bGlob_int"
     gene            1450..2973
                     /label="vsv-G"
                     /gene="vsv-G"
     CDS             1450..2985
                     /label="ORF frame 1"
     misc_feature    2950..2982
                     /label="VSVG Tag"
     rep_origin      complement(4055..4674)
                     /label="pBR322_origin"
     gene            complement(4829..5689)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4829..5689)
                     /label="ORF frame 1"
     promoter        complement(5731..5759)
                     /label="AmpR_promoter"
ORIGIN
    1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
   61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
  121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
  181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
  241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
  301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
  361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
  421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
  481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
  541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
  601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
  661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
  721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
  781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
  841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
  901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
  961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
 1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
 1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
 1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
 1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
 1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
 1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
 1381 ctggcccatc actttggcaa agcacgtgag atctgaattc aacagagatc gatctgtttc
 1441 cttgacacta tgaagtgcct tttgtactta gcctttttat tcattggggt gaattgcaag
 1501 ttcaccatag tttttccaca caaccaaaaa ggaaactgga aaaatgttcc ttctaattac
 1561 cattattgcc cgtcaagctc agatttaaat tggcataatg acttaatagg cacagccata
 1621 caagtcaaaa tgcccaagag tcacaaggct attcaagcag acggttggat gtgtcatgct
 1681 tccaaatggg tcactacttg tgatttccgc tggtatggac cgaagtatat aacacagtcc
 1741 atccgatcct tcactccatc tgtagaacaa tgcaaggaaa gcattgaaca aacgaaacaa
 1801 ggaacttggc tgaatccagg cttccctcct caaagttgtg gatatgcaac tgtgacggat
 1861 gccgaagcag tgattgtcca ggtgactcct caccatgtgc tggttgatga atacacagga
 1921 gaatgggttg attcacagtt catcaacgga aaatgcagca attacatatg ccccactgtc
 1981 cataactcta caacctggca ttctgactat aaggtcaaag ggctatgtga ttctaacctc
 2041 atttccatgg acatcacctt cttctcagag gacggagagc tatcatccct gggaaaggag
 2101 ggcacagggt tcagaagtaa ctactttgct tatgaaactg gaggcaaggc ctgcaaaatg
 2161 caatactgca agcattgggg agtcagactc ccatcaggtg tctggttcga gatggctgat
 2221 aaggatctct ttgctgcagc cagattccct gaatgcccag aagggtcaag tatctctgct
 2281 ccatctcaga cctcagtgga tgtaagtcta attcaggacg ttgagaggat cttggattat
 2341 tccctctgcc aagaaacctg gagcaaaatc agagcgggtc ttccaatctc tccagtggat
 2401 ctcagctatc ttgctcctaa aaacccagga accggtcctg ctttcaccat aatcaatggt
 2461 accctaaaat actttgagac cagatacatc agagtcgata ttgctgctcc aatcctctca
 2521 agaatggtcg gaatgatcag tggaactacc acagaaaggg aactgtggga tgactgggca
 2581 ccatatgaag acgtggaaat tggacccaat ggagttctga ggaccagttc aggatataag
 2641 tttcctttat acatgattgg acatggtatg ttggactccg atcttcatct tagctcaaag
 2701 gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt cgcaacttcc tgatgatgag
 2761 agtttatttt ttggtgatac tgggctatcc aaaaatccaa tcgagcttgt agaaggttgg
 2821 ttcagtagtt ggaaaagctc tattgcctct tttttcttta tcatagggtt aatcattgga
 2881 ctattcttgg ttctccgagt tggtatccat ctttgcatta aattaaagca caccaagaaa
 2941 agacagattt atacagacat agagatgaac cgacttggaa agtaactcaa atcctgcaca
 3001 acagattctt catgtttgga ccaaatcaac ttgtgatacc atgctcaaag aggcctcaat
 3061 tatatttgag tttttaattt ttatggaatt caccccacca gtgcaggctg cctatcagaa
 3121 agtggtggct ggtgtggcta atgccctggc ccacaagttt cactaagctc gcttccttgc
 3181 tgtccaattt ctattaaagg ttccttggtt ccctaagtcc aactactaaa ctgggggata
 3241 ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa
 3301 tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca
 3361 tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa
 3421 actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gccctgatgc
 3481 ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg gaggttaaag
 3541 tttggctatg ctgtatttta cattacttat tgttttagct gtcctcatga atgtcttttc
 3601 actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc tcttgcttag
 3661 agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg gtttctcctc
 3721 gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt gaagaaggaa
 3781 aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc cccactcaca
 3841 gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga tctgcttcct
 3901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
 3961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
 4021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
 4081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
 4141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
 4201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
 4261 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
 4321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
 4381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
 4441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
 4501 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
 4561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
 4621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
 4681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
 4741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
 4801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
 4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
 4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
 4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
 5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
 5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
 5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
 5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
 5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
 5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
 5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
 5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
 5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
 5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
 5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
 5701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
 5761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
 5821 acgt
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