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兔角膜上皮细胞SIRC BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: SIRC
Keywords: Rabbit Cornea
Cell Line Description: Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Species: Rabbit
Tissue of Origin: Cornea
CellType: Fibroblast
Growth Mode: Adherent
Karyotype: 2n = 44
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin 0.02% EDTA; 5% CO2, 37°C.
Culture Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Originator: No
Country: USA
References: Virus susceptibility: Science 1965;149:633; Proc Soc Exp Med 1966;122:783; Proc Soc Exp Med 1967;125:12
Additional Bibliography: Niederkorn JY1, Meyer DR, Ubelaker JE, Martin JH.,Ultrastructural and immunohistological characterization of the SIRC corneal cell line. In Vitro Cell Dev Biol. 1990 Sep;26(9):923-30. PMID:2228906
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
Cell Line Name: SIRC
Keywords: Rabbit Cornea
Cell Line Description: Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.
Species: Rabbit
Tissue of Origin: Cornea
CellType: Fibroblast
Growth Mode: Adherent
Karyotype: 2n = 44
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin 0.02% EDTA; 5% CO2, 37°C.
Culture Medium: EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Originator: No
Country: USA
References: Virus susceptibility: Science 1965;149:633; Proc Soc Exp Med 1966;122:783; Proc Soc Exp Med 1967;125:12
Additional Bibliography: Niederkorn JY1, Meyer DR, Ubelaker JE, Martin JH.,Ultrastructural and immunohistological characterization of the SIRC corneal cell line. In Vitro Cell Dev Biol. 1990 Sep;26(9):923-30. PMID:2228906
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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