Vaccinia virus vTF7-3牛痘病毒毒株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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Vaccinia virus vTF7-3
Classification: Poxviridae, Orthopoxvirus
Classification Poxviridae, Orthopoxvirus
Agent Vacciniavirus
Strain vTF7-3[Wr]
Applications Expresses bacteriophage T[7] RNA polymerase.
Expresses bacteriophage T[7] RNApolymerase.
Biosafety Level 2
Isolation Constructedin vitro
Product Format frozen
Storage Conditions -70°C
Comments Cellswhich have been infected with this construct can be transfected by plasmidswhich contain a gene targeted for expression inserted between a T7 promoter andT7 terminator.
Expresses bacteriophage T[7] RNApolymerase.
Recombinant virus constructed in vitro.
Expresses bacteriophage T[7] RNA polymerase.
Expression of the T7 RNA polymerase isdirected by the P7.5 promoter which includes early and late regulatory signals.
Constructed by transfecting vacciniavirus-infected cells with pTF703
Effect on Host Yes, in vitro effects: Cytopathic effects (plaque formation) onBS-C-1 and CV-1 monolayers
CPE, rounding, stringing, and celldestruction
Effect on Host Yes, in vitro effects: Cytopathic effects (plaque formation) onBS-C-1 and CV-1 monolayers
CPE, rounding, stringing, and celldestruction
Name of Depositor NIH, B Moss, NIH
U.S. Patent 5,550,035
U.S. Patent Number 5,550,035
Source Constructedin vitro
References FuerstTR, et al. Eukaryotic transient-expression system based on recombinant vacciniavirus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci.USA 83: 8122-8126, 1986. PubMed: 3095828
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